Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1493-CDB

R&D Systems, part of Bio-Techne
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1493-CDB-050
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Key Product Details

Source

NS0

Accession #

P25942-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD40/TNFRSF5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD40/TNFRSF5 protein
Human CD40
(Glu21-Arg193)
Accession # P25942-1		 DIEGRMD Human IgG1
(Pro100-Lys330)		 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu21

Predicted Molecular Mass

47 kDa 

SDS-PAGE

58-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD40/TNFRSF5 Fc Chimera is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal binding is 4-24 ng/mL.

Measured by its ability to inhibit rhCD40 Ligand-induced IL-8 secretion in HT1080 human fibrosarcoma cells transfected with human CD40.
The ED50 for this effect is 0.08-0.8 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 1493-CDB in the following applications:

Scientific Data Images for Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

Recombinant Human CD40/TNFRSF5 Fc Chimera Protein Bioactivity

Recombinant Human CD40/TNFRSF5 Fc Chimera Protein Bioactivity

When Recombinant Human CD40/TNFRSF5 Fc Chimera (Catalog # 1493-CDB) is immobilized at 1 µg/mL (100 µL/well), the concentration of Recombinant Human CD40 Ligand/TNFSF5 (Catalog # 6420-CL) that produces 50% optimal binding is 4-24 ng/mL.

Formulation, Preparation and Storage

1493-CDB
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD40/TNFRSF5

CD40, also known as TNFRSF5, is a 45-50 kDa type I transmembrane glycoprotein member of the TNF receptor superfamily (1). Mature human CD40 consists of a 173 amino acid (aa) extracellular domain, a transmembrane domain, and a 62 aa cytoplasmic domain (2). The extracellular domain of human CD40 shares 58% and 56% aa sequence identity with mouse and rat CD40, respectively. An antagonistic soluble human CD40 splice variant contains an alternate sequence within the extracellular and transmembrane domains and lacks a cytoplasmic domain (3). CD40 is expressed on the surface of B cells, dendritic cells, macrophages, monocytes and platelets, as well as endothelial and epithelial cells (4, 5). Interaction of CD40 with its ligand, CD40 Ligand, leads to the aggregation of CD40 molecules resulting in the initiation of bidirectional intracellular signaling in both CD40 and CD40 Ligand expressing cells (6). CD40 ligation by CD40 Ligand promotes B cell activation and T cell-dependent humoral responses (7, 8). CD40 serves multiple functions in both hematopoietic and epithelial cancers and is a target for tumor immunotherapy (9, 10). Dysregulation of CD40/CD40 Ligand expression and interactions contributes to the immune deficiency associated with HIV infection and AIDS (11, 12). It is also implicated in the pathology of multiple cardiovascular diseases including atherosclerosis, atherothrombosis, and restenosis (13, 14).

References

  1. Banchereau, J. et al. (1994) Annu. Rev. Immunol. 12:881.
  2. Stamenkovic, I. et al. (1989) EMBO J. 8:1403.
  3. Eshel, D. et al. (2008) Mol. Immunol. 46:250.
  4. van Kooten, C. and J. Banchereau (1997) Curr. Opin. Immunol. 9:330.
  5. Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
  6. Eissner, G. et al. (2004) Cytokine Growth Factor Rev. 15:353.
  7. Rickert, R.C. et al. (2011) Immunol. Rev. 244:115.
  8. Elgueta, R. et al. (2009) Immunol. Rev. 229:152.
  9. Loskog, A.S. and A.G. Eliopoulos (2009) Semin. Immunol. 21:301.
  10. Hangalapura, B.N. et al. (2012) J. Gene Med. 14:416.
  11. Kornbluth, R.S. (2000) J. Leukoc. Biol. 68:373.
  12. Chougnet, C. (2003) J. Leukoc. Biol. 74:702.
  13. Pamukcu, B. et al. (2011) Ann. Med. 43:331.
  14. Hassan, G.S. et al. (2012) Immunobiology 217:521.

Alternate Names

CD40, TNFRSF5

Entrez Gene IDs

958 (Human); 21939 (Mouse); 171369 (Rat); 102118696 (Cynomolgus Monkey)

Gene Symbol

CD40

UniProt

P25942-1

Additional CD40/TNFRSF5 Products

Product Documents for Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human CD40/TNFRSF5 Fc Chimera Protein, CF

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