Recombinant Human CD43 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9680-CD

R&D Systems, part of Bio-Techne
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9680-CD-050
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Key Product Details

Source

NS0

Accession #

P16150

Conjugate

Unconjugated

View all CD43 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CD43 protein
Ser20-Arg253, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser20

Predicted Molecular Mass

25 kDa

SDS-PAGE

77-88 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Human CD43 binds to Recombinant Human Siglec‑1/CD169 (Catalog # 5197-SL) with an ED50 of 1.00-10.0  μg/mL.

Scientific Data Images for Recombinant Human CD43 Protein, CF

Recombinant Human CD43 Protein Binding Activity

Recombinant Human CD43 Protein Binding Activity

Recombinant Human CD43 binds to Recombinant Human Siglec-1/CD169 (Catalog # 5197-SL) binds with an ED50 of 1.00-10.0 µg/mL.

Formulation, Preparation and Storage

9680-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD43

CD43, also known as Leukosialin and Sialophorin and Ly-48, is a type I transmembrane sialylated mucin that is expressed on most leukocytes and some tumor cells (1). Notably, the membrane expression of CD43 seems to be a characteristic of leukocytes, while cytoplasmic expression without membrane insertion occurs in endothelium and select epithelia. While CD43 restricts leukocyte adhesion and modulates T cell activation, these activities are context specific (2). CD43 can both induce and protect against apoptosis, and can either promote or block cell adhesion (3). In human, CD43 is synthesized as a 400 amino acid (aa) precursor that contains a 19 aa signal sequence, a 234 aa extracellular region, a 23 aa TM domain, and a 124 aa cytoplasmic tail. The extracellular region (aa 20-253) has 25 O-linked glycosylation sites and 1 N-linked glycosylation site. Human CD43 extracellular region has less than 60% aa identity to the extracellular regions in rat and mouse.

References

  1. Santamaría, M. et al. (1996). Cancer Res. 56:3526.
  2. Manjunath, N. et al. (1995) Nature 377:535.
  3. Brown, T. J. et al. (1996) J. Biol. Chem. 271:27686.

Long Name

Cluster of Differentiation 43

Alternate Names

CD43, GPL115, Leukosialin, LSN, Ly-48, Sialophorin, SPN

Entrez Gene IDs

6693 (Human); 20737 (Mouse); 24796 (Rat); 102147271 (Cynomolgus Monkey)

Gene Symbol

SPN

UniProt

P16150

Additional CD43 Products

Product Documents for Recombinant Human CD43 Protein, CF

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Product Specific Notices for Recombinant Human CD43 Protein, CF

For research use only

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