Recombinant Human CD44v5 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI10146

Biotinylated
R&D Systems, part of Bio-Techne
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AVI10146-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

NP_001001391.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD44 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD44 protein

Human CD44v5
(Gln21-Thr222) NVDRNGTTAYEGNWNPEAHPPLIHHEHHEEEETPHSTST (Arg223-Trp269)
Accession # P16070-12

 IEGRMD Human IgG1
(Pro100-Lys330		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln21 inferred from enzymatic pyroglutamate treatment revealing Ile22.

Predicted Molecular Mass

60 kDa

SDS-PAGE

100-120 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When 2.00 μg/mL of Biotinylated Recombinant Human CD44v5 Fc Chimera Avi-tag protein is immobilized onto Streptavidin coated plate (Catalog # CP004) Recombinant Human LSECtin/CLEC4G  (Catalog # 2947-CL) binds with an ED50 of 10.0-60.0 ng/mL.

Scientific Data Images for Recombinant Human CD44v5 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human CD44 Fc Chimera Avi-tag Protein Binding Activity.

When 2.00 μg/mL of Biotinylated Recombinant Human CD44v5 Fc Chimera Avi-tag Protein (Catalog # AVI10146) is immobilized onto Streptavidin coated plate (CP004), Recombinant Human LSECtin/CLEC4G (2947-CL) binds with an ED50 of 10.0-60.0 ng/mL.

Biotinylated Recombinant Human CD44 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD44 Fc Chimera Avi-tag Protein (Catalog # AVI10146) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 100-120 kDa and 200-240 kDa, respectively.

Formulation, Preparation and Storage

AVI10146
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD44

CD44, also known as extracellular matrix receptor III (ECMRIII), is a non-kinase transmembrane glycoprotein that regulates numerous functions including cell proliferation, adhesion, migration, hematopoiesis, and lymphocyte activation. Mature human CD44 consists of an extracellular domain (ECD) with a hyaluronan-binding, disulfide-stabilized link region and a stem region, a transmembrane domain, and a cytoplasmic domain. The full-length ECD of human CD44 shares 63% amino acid sequence identity with mouse CD44. In humans, CD44 is encoded by 19 exons with 10 constant in all isoforms. The standard form of CD44 (CD44s) is encoded by the 10 constant exons and is the shortest and most abundantly expressed isoform. The remaining 9, variant exons of CD44 can undergo complex alternative splicing and are expressed in a cell-specific manner and in the pathophysiology of many diseases. The N-terminal ECD of CD44 contains a binding site for Hyaluronan (HA), the most common ligand of CD44. CD44 exhibits multiple different functions and is aberrantly up-regulated among diverse tumors. It can bind HA and function as a "platform" for growth factors and metalloproteinases. CD44 can function as a co-receptor that modifies activity of receptors including MET and the ERBB family of tyrosine kinases. Further, CD44 can link the plasma membrane to the actin cytoskeleton via the ERM. CD44 has been found to bind E- and L-selectin on tumor cells and E-selectin on neutrophils. Additionally, distinct variants of CD44 have been found to interact with LSECtin and potentially mediate its function in the immune system. Human CD44v5 contains exon 10 and is associated with tumor progression and metastasis in many cancer types and is considered a functional cancer biomarker. Our Avi-tag Biotinylated CD44 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Ponta, H. et al. (2003) Nat. Rev. Mol. Cell Biol. 4:33.
  2. Senbanjo, L.T. and Chellaiah, M.A. (2017) Front Cell Dev Biol. 5:18.
  3. Chen, C. at al. (2018) J Hematol Oncol 11:64.
  4. Xu, H. et al. (2020) Hematol Oncol 9:36.
  5. Nagano, O. and H. Saya (2004) Cancer Sci. 95:930.
  6. Hanley, W.D. et al. (2005) Cancer Res. 65:5812.
  7. Katayama, Y. et al. (2005) J. Exp. Med. 18:1183.
  8. Tang, L. et al. (2010) Eur. J. Immunol. 40:1185.

Alternate Names

CD44, ECMR-III, HCAM, HCELL, LHR, MDU2, MDU3, MIC4, MUTCH-I, Pgp1

Entrez Gene IDs

960 (Human); 12505 (Mouse); 25406 (Rat); 100126860 (Porcine)

Gene Symbol

CD44

UniProt

NP_001001391.1

Additional CD44 Products

Product Documents for Recombinant Human CD44v5 Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CD44v5 Fc Chimera Avi-tag Protein, CF

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