Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI3644

Biotinylated
R&D Systems, part of Bio-Techne
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

CHO

Accession #

P09326.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD48/SLAMF2 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD48/SLAMF2 protein
Human CD48/SLAMF2
(Gln27-Ser220)
Accession # P09326.2		 HHHHHH Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln27 (Blocked, Predicted)

Predicted Molecular Mass

25 kDa

SDS-PAGE

43-51 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (Catalog # 1039-2B) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag (Catalog # AVI3644) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein Binding Activity.

When Recombinant Human 2B4/CD244/SLAMF4 Fc Chimera (1039-2B) is immobilized at 1 µg/mL (100 µL/well), the concentration of Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag (Catalog # AVI3644) that produces 50% of the optimal binding response is approximately 0.3-1.8 μg/mL.

Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD48/SLAMF2 His-tag Avi-tag (Catalog # AVI3644) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 43-51 kDa.

Formulation, Preparation and Storage

AVI3644
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD48/SLAMF2

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily proteins (1-3). The human CD48 cDNA encodes a 243 amino acid (aa) precursor that includes a 26 aa signal sequence, a 194 aa mature protein containing two Ig-like C2-type domains, and a 23 aa C-terminal propeptide (4). A soluble form of CD48 has been detected in the serum of lymphoid leukemia and arthritis patients (5). Human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. It shares 20%-26% aa sequence identity with comparable regions of human 2B4, BLAME, CD2F-10, CD84, CD229, CRACC, NTB-A, and SLAM. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors (4, 6). Among dendritic cells (DC), CD48 is selectively expressed on circulating myeloid DC and resident bone marrow and thymus DC (7). CD2, 2B4, and heparan sulfate function as CD48 ligands (8-10). CD48 is competent to transduce signals and can also trigger signaling through CD2 or 2B4 (8, 11). CD48-CD2 interactions promote T cell activation and class switching to IgG2a in B cells (8, 12). High affinity CD48-2B4 interactions can either promote or inhibit NK cell and cytotoxic T cell (CTL) activation (7, 11, 13, 14). CD48-2B4 ligation does not directly trigger CTL activity but enhances signaling from the T cell receptor (13). CD48-2B4 mediated inhibition of NK cell activity is distinct from MHC I-restricted mechanisms (15). CD48 expressed on NK cells is co-activating, whereas CD48 expressed on other cell types inhibits NK cell activation (14). Both CD48 expressing and non-expressing cells can be targets of NK cell or CTL-mediated lysis (13, 16). Our Avi-tag Biotinylated human CD48 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. McArdel, S.L. et al. (2016) Clin. Immunol. 164:10.
  2. Bhat, R. et al. (2006) J. Leukocyte Biol. 79:417.
  3. Loertscher, R. and Lavery, P. (2002) Transpl. Immunol. 9:93.
  4. Wong, Y.W. et al. (1990) J. Exp. Med. 171:2115.
  5. Smith, G.M. et al. (1997) J. Clin. Immunol. 17:502.
  6. Kiel, M.J. et al. (2005) Cell 121:1109.
  7. Morandi, B. et al. (2005) J. Immunol. 175:3690.
  8. Kato, K. et al. (1992) J. Exp. Med. 176:1241.
  9. Latchman, Y. et al. (1998) J. Immunol. 161:5809.
  10. Ianelli, C.J. et al. (1998) J. Biol. Chem. 273:23367.
  11. Messmer, B. et al. (2006) J. Immunol. 176:4646.
  12. Gao, N. et al. (2005) J. Immunol. 174:4113.
  13. Lee, K-M. et al. (2003) J. Immunol. 170:4881.
  14. Lee, K-M. et al. (2006) Blood 107:3181.
  15. McNerney, M.E. et al. (2005) Blood 106:1337.
  16. Lee, K-M. et al. (2004) J. Exp. Med. 199:1245.

Long Name

SLAM Family Member 2

Alternate Names

BCM1, BLAST1, CD48, MEM-102, SLAMF2

Entrez Gene IDs

962 (Human); 12506 (Mouse); 245962 (Rat); 102134608 (Cynomolgus Monkey)

Gene Symbol

CD48

UniProt

P09326.2

Additional CD48/SLAMF2 Products

Product Documents for Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CD48/SLAMF2 His-tag Avi-tag Protein, CF

For research use only

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