Recombinant Human CD5 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11369

Biotinylated
R&D Systems, part of Bio-Techne
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AVI11369-050
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Key Product Details

Source

HEK293

Accession #

P06127.2

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD5 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CD5 protein
Human CD5
(Arg25-Asn371)
Accession # P06127.2		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg25

Predicted Molecular Mass

67 kDa

SDS-PAGE

77-85 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
When Human CD5 Antibody (Catalog # MAB16361) is immobilized at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human CD5 Fc Chimera Avi-tag (Catalog # AVI1636) binds with an ED50 of 0.800-8.00 ng/mL.

Scientific Data Images for Recombinant Human CD5 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human CD5 Fc Chimera Avi-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. When Human CD5 Antibody (MAB16361) is immobilized at 0.500 µg/mL (100 µL/well), Biotinylated Recombinant Human CD5 Fc Chimera Avi-tag Protein (Catalog # AVI11369) binds with an ED50 of 0.800-8.00 ng/mL.

Biotinylated Recombinant Human CD5 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD5 Fc Chimera Avi-tag Protein (Catalog # AVI11369) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 77-85 kDa and 150-170 kDa, respectively.

Formulation, Preparation and Storage

AVI11369
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD5

CD5, also known as Leu-1, Ly-1, and T1, is a 67 kDa transmembrane glycoprotein in the scavenger receptor superfamily (1). Mature human CD5 consists of a 348 amino acid (aa) extracellular domain (ECD) with three scavenger receptor cysteine-rich (SRCR) domains, a 30 aa transmembrane segment, and a 93 aa cytoplasmic domain (2). Within the ECD, human CD5 shares 55% aa sequence identity with mouse and rat CD5. The 52 kDa ECD can be cleaved from the cell surface and circulates in the serum (3). CD5 has been shown to interact homophilically, with CD72 on B cells, and with beta-glucan components of fungal cell walls (4‑6). CD5 expression on developing thymocytes is positively regulated by signaling through the T cell antigen receptor (TCR) and is up‑regulated on tolerized peripheral CD4+ cells (7, 8). It inhibits TCR signaling and promotes T cell nonresponsiveness and survival (8‑10). CD5 signaling inhibits the generation of regulatory T cells but promotes the development of Th17 cells (11, 12). Within the B cell lineage, CD5 is expressed on B-1a cells, anergic B cells, and IL-10 producing regulatory B cells (13‑16). Similarly to on T cells, it negatively regulates signaling through the B cell antigen receptor and supports peripheral B cell survival, anergy, and tolerance (13, 14, 16). B cells can produce an intracellularly-retained form of CD5 which lacks the signal peptide and a portion of the first SRCR domain (17). CD5 is also involved in the cellular entry of hepatitis C virus into T cells (18). Our Avi-tag Biotinylated Human CD5 His tag protein features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Soldevila, G. et al. (2011) Curr. Opin. Immunol. 23:310.
  2. Jones, N.H. et al. (1986) Nature 323:346.
  3. Calvo, J. et al. (1999) Tissue Antigens 54:128.
  4. Brown, M.H. and E. Lacey (2010) J. Immunol. 185:6068.
  5. Van de Velde, H. et al. (1991) Nature 351:662.
  6. Vera, J. et al. (2009) Proc. Natl. Acad. Sci. 106:1506.
  7. Azzam, H.S. et al. (1998) J. Exp. Med. 188:2301.
  8. Hawiger, D. et al. (2004) Immunity 20:695.
  9. Tarakhovsky, A. et al. (1995) Science 269:535.
  10. Friedlein, G. et al. (2007) J. Immunol. 178:6821.
  11. Ordonez-Rueda, D. et al. (2009) Eur. J. Immunol. 39:2233.
  12. de Wit, J. et al. (2011) Blood 118:6107.
  13. Bikah, G. et al. (1996) Science 274:1906.
  14. Hippen, K.L. et al. (2000) J. Exp. Med. 191:883.
  15. Yanaba, K. et al. (2008) Immunity 28:639.
  16. Gary-Gouy, H. et al. (2002) Blood 100:4537.
  17. Renaudineau, Y. et al. (2005) Blood 106:2781.
  18. Sarhan, M.A. et al. (2012) J. Virol. Epub.

Alternate Names

CD5

Entrez Gene IDs

921 (Human); 12507 (Mouse)

Gene Symbol

CD5

UniProt

P06127.2

Additional CD5 Products

Product Documents for Recombinant Human CD5 Fc Chimera Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human CD5 Fc Chimera Avi-tag Protein, CF

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