Recombinant Human CD63 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5048-CD

R&D Systems, part of Bio-Techne
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5048-CD-050
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Key Product Details

Source

CHO

Accession #

P08962-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD63 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD63 protein
MD
 Human IgG1
(Pro100-Lys330)		 IEGR Human CD63
(Ala103-Val203)
Accession # P08962-1
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

38 kDa

SDS-PAGE

41-60 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human BST-2/Tetherin (Catalog # 9939-BS) is immobilized at 2.5 μg/mL (100 μL/well), Recombinant CD63 Fc Chimera binds with an ED50 of 1-6 μg/mL.   

Scientific Data Images for Recombinant Human CD63 Fc Chimera Protein, CF

When Recombinant Human BST-2/Tethrin (9939-BS) is immodibilized at 2.5 μg/mL, 100 μL/well, Recombinant Human CD63 Fc Chimera (Catalog # 5048-CD) binds with an ED50 of 1-6 μg/mL.
Recombinant Human CD63 Fc Chimera Protein SDS-PAGE

Recombinant Human CD63 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human CD63 Fc Chimera was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 41-60 kDa and 80-120 kDa, respectively.

Formulation, Preparation and Storage

5048-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: CD63

CD63, also known as Tspan-30, is a member of the tetraspanin family of proteins that mediate signal transduction events which play a role in the regulation of cell development, activation, growth and motility (1, 2). These membrane proteins are characterized by the presence of four transmembrane domains, a small extracellular loop (SEL) and a large extracellular loop (LEL) (2, 3). Regions of the tetraspanin LEL are known to associate with integrins to regulate membrane protein trafficking, leukocyte recruitment, and adhesion processes (4, 5). Deficiency of CD63 is associated with Hermansky-Pudlak syndrome (6). Human CD63-LEL shares 67% and 66% aa sequence identity with mouse and rat CD63-LEL, respectively.  CD63 has been identified as a cell surface binding partner for tissue inhibitor of metalloproteinase 1 (TIMP-1), modulating the tetraspanin/integrin signaling complex which regulates cell survival and polarization (7).

References

  1. Kang, M. et al. (2014) PLOS One. 9:e102817.
  2. Yu, M. et al. (2016) J. Immunol. Res. 2016:1.
  3. Chaiyadet, S. et al. (2017) Sci. Rep 7:14342.
  4. Tugues, S. et al. (2013) J. Biol. Chem. 288:19060.
  5. Yañez-Mo, M. et al. (2009) Trends Cell Biol. 19:434.
  6. Nishibori, M. et al. (1993) J. Clin. Invest. 91:1775.
  7. Jung, K.K. et al. (2006) EMBO J. 25:3934.

Alternate Names

CD63, Granulophysin, Lamp-3, ME491, OMA81H, Tspan30

Entrez Gene IDs

967 (Human); 12512 (Mouse); 29186 (Rat); 474391 (Canine)

Gene Symbol

CD63

UniProt

P08962-1

Additional CD63 Products

Product Documents for Recombinant Human CD63 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human CD63 Fc Chimera Protein, CF

For research use only

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