Recombinant Human CD81 LEL Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9144-CD

R&D Systems, part of Bio-Techne
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Key Product Details

Source

CHO

Accession #

P60033

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CD81 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD81 protein
MD Human IgG1
(Pro100-Lys330)		 IEGR

Human CD81 LEL
(Phe113-Lys201)
Accession # P60033
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

36 kDa

SDS-PAGE

36-41 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CD81 LEL Fc Chimera is used at 2 μg/mL, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2-1.2 µg/mL.

Scientific Data Images for Recombinant Human CD81 LEL Fc Chimera Protein, CF

Recombinant Human CD81 LEL Fc Chimera Protein Bioactivity

Recombinant Human CD81 LEL Fc Chimera Protein Bioactivity

When Recombinant Human CD81 LEL Fc Chimera (Catalog # 9144-CD) is used at 2 µg/mL, Recombinant Viral HCV E2 (Catalog # 9146-HC) binds with an ED50 of 0.2-1.2 µg/mL.

Formulation, Preparation and Storage

9144-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD81

CD81, also known as TAPA-1 and Tetraspanin-28, is an approximately 25 kDa palmitoylated component of plasma membrane lipid rafts (1). It contains four transmembrane segments, two extracellular loops of 30 and 90 amino acids (aa), and three short cytoplasmic regions (2, 3). Within the large extracellular loop, human CD81 shares 90% and 84% aa sequence identity with mouse and rat CD81, respectively. CD81 associates with a wide range of membrane proteins including CD151, TfR2, LDL R, PCSK9, Glypican 3, IFITM1, IGSF8/CD316, FPRP, and complexes of CD19-CD21 (4-11). It is required for the development of CD4+CD8+ DP thymocytes (12) and hepatocyte infection by Plasmodium sporozoites (13). It also supports B cell receptor signaling (11), Hepcidin expression (5), monocyte and B cell tethering to the vascular endothelium (14), and the immunosuppressive function of Treg and MDSC (15). CD81 additionally functions as a receptor for the E2 glycoprotein of hepatitis C virus (16). The CD81-E2 interaction inhibits NK cell cytolytic activity, provides a co-stimulatory signal to T cells, and inhibits the maturation of plasmacytoid dendritic cells (17-19).

References

  1. Charrin, S. et al. (2014) J. Cell Sci. 127:3641.
  2. Oren, R. et al. (1990) Mol. Cell. Biol. 10:4007.
  3. Levy, S. et al. (1991) J. Biol. Chem. 266:14597.
  4. Zhu, Y.-Z. et al. (2012) Virology 429:112.
  5. Chen, J. and C.A. Enns (2015) J. Biol. Chem. 290:7841.
  6. Le, Q.-T. et al. (2015) J. Biol. Chem. 290:23385.
  7. Takahashi, S. et al. (1990) J. Immunol. 145:2207.
  8. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:40545.
  9. Stipp, C.S. et al. (2001) J. Biol. Chem. 276:4853.
  10. Liu, B. et al. (2009) Am. J. Pathol. 175:717.
  11. Cherukuri, A. et al. (2004) J. Immunol. 279:31973.
  12. Boismenu, R. et al. (1996) Science 271:198.
  13. Silvie, O. et al. (2003) Nat. Med. 9:93.
  14. Feigelson, S.W. et al. (2003) J. Biol. Chem. 278:51203.
  15. Vences-Catalan, F. et al. (2015) Cancer Res. 75:4517.
  16. Pileri, P. et al. (1998) Science 282:938.
  17. Crotta, S. et al. (2002) J. Exp. Med. 195:35.
  18. Tseng, C.-T. and G.R. Klimpel (2002) J. Exp. Med. 195:43.
  19. Tu, Z. et al. (2013) Cell. Immunol. 284:98.

Alternate Names

CD81, TAPA-1, TSPAN28

Entrez Gene IDs

975 (Human); 12520 (Mouse); 25621 (Rat)

Gene Symbol

CD81

UniProt

P60033

Additional CD81 Products

Product Documents for Recombinant Human CD81 LEL Fc Chimera Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CD81 LEL Fc Chimera Protein, CF

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