Recombinant Human CD96 Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 9360-CD
Key Product Details
Source
HEK293
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human CD96 protein
Human CD96 (Lys25-Met519) Accession # P40200-1 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
N-terminus | C-terminus |
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Lys25
Predicted Molecular Mass
81 kDa
SDS-PAGE
131 - 160 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 Fc Chimera that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.
When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 μg/mL, 100 μL/well, the concentration of Recombinant Human CD96 Fc Chimera that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.
Scientific Data Images for Recombinant Human CD96 Fc Chimera Protein, CF
Recombinant Human CD96 Fc Chimera Protein Bioactivity
When Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) is immobilized at 1 µg/mL, 100 µL/well, Recombinant Human CD96 Fc Chimera (Catalog # 9360-CD) binds with an ED50 of 0.3-1.8 µg/mL.Formulation, Preparation and Storage
9360-CD
Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
Reconstitution |
Reconstitute at 500 μg/mL in PBS.
|
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CD96
References
- Wang, P.L. et al. (1992) J. Immunol. 148:2600.
- Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
- Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
- Xie, W. et al. (2010) Cytometry A 77:840.
- Eriksson, E. M. et al. (2012) PLOS One 7:e51696.
- Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
- Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
- Protein Accession # BAE32358.
- Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
- Fuchs, A. et al. (2004) J. Immunol. 172:3394.
- Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
- Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.
Alternate Names
CD96, TACTILE
Gene Symbol
CD96
UniProt
Additional CD96 Products
Product Documents for Recombinant Human CD96 Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human CD96 Fc Chimera Protein, CF
For research use only
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