Recombinant Human CD96v2 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9556

R&D Systems, part of Bio-Techne
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AVI9556-050
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Key Product Details

Source

CHO

Accession #

NP_005807.1

Structure / Form

Disulfide-linked homodimer
Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CD96 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CD96 protein
Human CD96v2
(Lys25-Met503)
Accession # NP_005807.1		 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys25

Predicted Molecular Mass

82 kDa

SDS-PAGE

117-146 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag (Catalog # AVI9556) binds Recombinant Human CD155/PVR Fc Chimera (Catalog # 9174-CD) with an ED50 of 30.0-300 ng/mL.

Scientific Data Images for Recombinant Human CD96v2 Fc Chimera Avi-tag Protein, CF

Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein Binding Activity.

Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein (Catalog # AVI9556) binds Recombinant Human CD155/PVR Fc Chimera (9174-CD) with an ED50 of 30.0-300 ng/mL.

Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CD96v2 Fc Chimera Avi-tag Protein (Catalog # AVI9556) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 117-146 kDa, and 230-290 kDa, respectively.

Formulation, Preparation and Storage

AVI9556
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD96

Human CD96, also known as Tactile (T cell-activated increased late expression), is a 160 kDa type I transmembrane glycoprotein of the Ig superfamily that shows peak expression 6-9 days after activation of T, NK, and a subpopulation of B cells (1, 2). CD96 is also frequently expressed on acute myeloid leukemia and myelodysplastic stem cells (3, 4). It is expressed on CD4+ and CD8+ T cells, plus NK cells and select B cells (5). Low expression of adhesive human CD69 is a rare cause of C syndrome, a set of developmental anomalies in cranial bone (trigonocephaly), skin and viscera, demonstrating a role for CD96 in development of these tissues (2, 6). Human CD96 has two isoforms generated by alternative splicing. Isoform 2 is the most common form of CD96 that shows a 16 amino acid (aa) deletion comparing to isoform 1. Mature human CD96 isoform 2 is a 548 amino acid (aa), type I transmembrane glycoprotein. It contains a 482 aa extracellular region that contains three Ig-like domains. The two N-terminal domains are V-type and I-type, while the distal domain is a C-type structure (2). Within the ECD, human CD96 shares 53% and 52% aa sequence identity with mouse CD96 and rat CD96, respectively (7). The ECD is highly N- and O-glycosylated (1). An 80 kDa soluble form is elevated in human serum during chronic hepatitis B (9). The most N-terminal Ig-like domain of human CD96 binds to CD155/PVR (poliovirus receptor), but CD96/CD155 interaction is species-specific (2, 7, 10). Mouse CD96 also binds Nectin-1 (7). On NK cells, costimulatory CD96 and DNAM-1 (CD226) are thought to have paired activity with inhibitory TIGIT, all of which bind CD155 and various Nectins (11, 12). CD96 can promote NK cell adhesion to, and killing of target cells, including tumors that express CD155 (10, 11). Our Avi-tag Biotinylated CD96 Fc Chimera features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Wang, P.L. et al. (1992) J. Immunol. 148:2600.
  2. Meyer, D. et al. (2009) J. Biol. Chem. 284:2235.
  3. Hosen, N. et al. (2007) Proc. Natl. Acad. Sci. USA 104:11008.
  4. Xie, W. et al. (2010) Cytometry A 77:840.
  5. Eriksson,E. M. et al. (2012) PLOS One 7:e51696
  6. Kaname, T. et al. (2007) Am. J. Hum. Genet. 81:835.
  7. Seth, S. et al. (2007) Biochem. Biophys. Res. Commun. 364:959.
  8. Protein Accession # BAE32358.
  9. Gong, J. et al. (2008) Clin. Exp. Immunol. 155:207.
  10. Fuchs, A. et al. (2004) J. Immunol. 172:3394.
  11. Stanietsky, N. and O. Mandelboim (2010) FEBS Lett. 584:4895.
  12. Xu, Z. and B. Jin (2010) Cell. Mol. Immunol. 7:11.

Alternate Names

CD96, TACTILE

Entrez Gene IDs

10225 (Human); 84544 (Mouse); 498079 (Rat); 102121080 (Cynomolgus Monkey)

Gene Symbol

CD96

UniProt

NP_005807.1

Additional CD96 Products

Product Documents for Recombinant Human CD96v2 Fc Chimera Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Recombinant Human CD96v2 Fc Chimera Avi-tag Protein, CF

For research use only

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