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Recombinant Human CDC25B (aa 377-566) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 1649-CD

R&D Systems, part of Bio-Techne
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1649-CD-050

Key Product Details

Source

E. coli

Accession #

Structure / Form

Based on mass spectrometric analysis, a carboxyl-terminal truncated peptide with a molecular mass of approximately 22.1 kDa may also be present in the recombinant protein preparation.

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

E. coli-derived human CDC25B protein
Glu377-Gln566, with an N-terminal Met and 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

23 kDa

SDS-PAGE

23-25 kDa, reducing conditions

Activity

Measured by its ability to cleave a substrate, p-Nitrophenyl phosphate (pNPP).
The specific activity is >70 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

1649-CD
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CDC25B

Cell Division Cycle 25B (Cdc25B) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Breast cancer patients bearing tumors containing high levels of Cdc25B have been found to have a greater incidence of aggressive, high‑grade tumors than those with low Cdc25B levels (2). In cells, the levels of Cdc25B activity are highest during the G2/M transition of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Overexpression of Cdc25B reduces the G2/M cell cycle block caused by ionizing radiation (4). Although activated by phosphorylation, Ser323 phosphorylation causes the enzyme to bind the protein 14-3-3, preventing substrate access to the catalytic site (5). One of the major substrates of Cdc25B is Cdc2, a kinase that is activated by dephosphorylation (6). The recombinant protein is truncated to remove the N-terminal regulatory domains and is fully active.

References

  1. Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta 1332:M53.
  2. Galaktionov, K. et al. (1995) Science 269:1575.
  3. Lammer, C. et al. (1998) J. Cell Sci. 111:2445.
  4. Miyata, H. et al. (2001) Cancer Res. 61:3188.
  5. Forrest, A. and B. Gabrielli (2001) Oncogene 20:4393.
  6. Gautier, J. et al. (1991) Cell 67:197.

Long Name

Cell Division Cycle 25B

Alternate Names

CDC25HU2, cell division cycle 25 homolog B (S. cerevisiae), cell division cycle 25 homolog B (S. pombe), cell division cycle 25B, Dual specificity phosphatase Cdc25B, EC 3.1.3.48, M-phase inducer phosphatase 2

Entrez Gene IDs

994 (Human)

Gene Symbol

CDC25B

UniProt

Additional CDC25B Products

Product Documents for Recombinant Human CDC25B (aa 377-566) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human CDC25B (aa 377-566) Protein, CF

For research use only

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