Recombinant Human CDC25B (aa 377-566) Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1649-CD
Key Product Details
Source
Accession #
Structure / Form
Conjugate
Applications
Product Specifications
Source
Glu377-Gln566, with an N-terminal Met and 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
SDS-PAGE
Activity
The specific activity is >70 pmol/min/μg, as measured under the described conditions.
Formulation, Preparation and Storage
1649-CD
Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl, EDTA, DTT and Glycerol. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: CDC25B
Cell Division Cycle 25B (Cdc25B) phosphatase removes inorganic phosphate groups covalently attached to tyrosine, serine and threonine residues in proteins (1). Breast cancer patients bearing tumors containing high levels of Cdc25B have been found to have a greater incidence of aggressive, high‑grade tumors than those with low Cdc25B levels (2). In cells, the levels of Cdc25B activity are highest during the G2/M transition of the cell cycle, where it is suspected to be involved in “checkpoint” control of cell cycle progression (3). Overexpression of Cdc25B reduces the G2/M cell cycle block caused by ionizing radiation (4). Although activated by phosphorylation, Ser323 phosphorylation causes the enzyme to bind the protein 14-3-3, preventing substrate access to the catalytic site (5). One of the major substrates of Cdc25B is Cdc2, a kinase that is activated by dephosphorylation (6). The recombinant protein is truncated to remove the N-terminal regulatory domains and is fully active.
References
- Draetta, G. and J. Eckstein (1997) Biochim. Biophys. Acta 1332:M53.
- Galaktionov, K. et al. (1995) Science 269:1575.
- Lammer, C. et al. (1998) J. Cell Sci. 111:2445.
- Miyata, H. et al. (2001) Cancer Res. 61:3188.
- Forrest, A. and B. Gabrielli (2001) Oncogene 20:4393.
- Gautier, J. et al. (1991) Cell 67:197.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional CDC25B Products
Product Documents for Recombinant Human CDC25B (aa 377-566) Protein, CF
Product Specific Notices for Recombinant Human CDC25B (aa 377-566) Protein, CF
For research use only