Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10328-CU

R&D Systems, part of Bio-Techne
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10328-CU-050
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Key Product Details

Source

HEK293

Accession #

Q9H5V8-3

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CDCP1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CDCP1 protein
Human CDCP1
(Phe30-Glu343)
Accession # Q9H5V8-3		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Phe30

Predicted Molecular Mass

62 kDa

SDS-PAGE

75-95 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CDCP1 Fc Chimera (Catalog # 10328-CU) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human P-Cadherin Fc Chimera (Catalog # 861-PC) binds with an ED50 of 0.75-6 μg/mL.

Scientific Data Images for Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF

Recombinant Human CDCP1 (Secreted Form) Fc Protein Binding Activity

Recombinant Human CDCP1 (Secreted Form) Fc Protein Binding Activity

When Recombinant Human CDCP1 Fc Chimera (Catalog # 10328-CU) is immobilized at 2 µg/mL (100 µL/well), Recombinant Human P-Cadherin Fc Chimera (Catalog # 861-PC) binds with an ED50 of 0.75-6 µg/mL.
Recombinant Human CDCP1 (Secreted Form) Fc Protein SDS-PAGE

Recombinant Human CDCP1 (Secreted Form) Fc Protein SDS-PAGE

2 μg/lane of Recombinant Human CDCP1 (Secreted Form) Fc Chimera (Catalog # 10328-CU) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 75-95 kDa and 150-190 kDa, respectively.

Formulation, Preparation and Storage

10328-CU
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CDCP1

CDCP1 (CUB-domain containing protein 1), also known as Trask or CD318, is a type I transmembrane receptor whose specific role remains unclear. Human CDCP1 is synthesized with a large extracellular domain (ECD) containing three CUB domains, a transmembrane domain and a cytoplasmic domain with 5 tyrosine phosphorylation sites (1). Full-length CDCP1 is ~135 kDa and can be cleaved into a ~70 kDa membrane-bound and a ~65 kDa circulating form (2). A secreted only form of human CDCP1, also called Isoform 2, can also be expressed consisting of amino acids (aa) 30-343 of the ECD (3). Mature human Isoform 2 shares 86% and 85% aa sequence identity to mouse and rat CDCP1 Isoform 2, respectively. CDCP1 was originally identified from proteins involved in metastasis and has been found on tumor, stem cells, keratinocytes and colonic epithelial cells (1). Tyrosine phosphorylation of the intracellular domain of CDCP1 results in downstream signaling through Src-family kinases (SFKs), Akt, and PKC delta (2, 4, 5). CDCP1 appears to be important for cell-cell and cell-substratum adhesion and the phosphorylation state of CDCP1 regulates this effect (5, 7). CDCP1 is associated with a poor prognosis in epithelial tumors, such as lung, pancreatic, colorectal, renal, and ovarian carcinomas (rEf) and is a novel marker of the most aggressive human triple-negative breast cancers (7, 8). Several different extracellular forms of CDCP1 have been shown to exhibit disease-specific expression in prostate cancer (9).

References

  1. Hooper, J.D. et al. (2003) Oncogene 22:1783.
  2. He, Y. et al. (2010) J. Biol. Chem. 285:26162.
  3. Perry, S.E. (2007) FEBS Lett. 581:1137.
  4. Law, M.E. et al. (2016) Breast Cancer Res. 18:80.
  5. Casar B. et al. (2012) Oncogene 31:3924.
  6. Spassov, D.S. et al. (2013) Cancer Res. 73:1168.
  7. Turdo, F. et al. (2016) Oncotarget 7:69649.
  8. Wright, H.J. et al. (2016) Oncogene 35:4762.
  9. Yang, L. et al. (2015) Oncotarget 6:43743.

Long Name

CUB Domain Containing Protein 1

Alternate Names

CD318, SIMA135

Entrez Gene IDs

64866 (Human); 109332 (Mouse)

Gene Symbol

CDCP1

UniProt

Q9H5V8-3

Additional CDCP1 Products

Product Documents for Recombinant Human CDCP1 (Secreted Form) Fc Protein, CF

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