Recombinant Human Cerberus 1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9515-CB

R&D Systems, part of Bio-Techne
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9515-CB-025
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Key Product Details

Source

T. ni (baculovirus)

Accession #

O95813

Conjugate

Unconjugated

Applications

Bioactivity

View all Cerberus 1 Proteins and Enzymes »

Product Specifications

Source

Trichoplusia ni, T. ni (baculovirus)-derived human Cerberus 1 protein
Human Cerberus 1
(Thr18-Ala267)
Accession # O95813		 HIEGR GGGSGGGSGGGS HHHHHHHHHH
N-terminus C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Thr18

Predicted Molecular Mass

31 kDa

SDS-PAGE

36-41 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Cerberus 1 (Catalog # 9515-CB) is coated at 2 µg/mL, Recombinant Human Nodal (Catalog # 3218-ND) binds with an ED50 = 0.4-2.4 ng/mL.

Scientific Data Images for Recombinant Human Cerberus 1 Protein, CF

Recombinant Human Cerberus 1 Protein Bioactivity

Recombinant Human Cerberus 1 Protein Bioactivity

When Recombinant Human Cerberus 1 (Catalog # 9515-CB) is coated at 2 µg/mL, Recombinant Human Nodal (Catalog # 3218-ND) binds with an ED50 = 0.4-2.4 ng/mL.

Formulation, Preparation and Storage

9515-CB
Formulation Lyophilized from a 0.2 μm filtered solution in HCl with Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cerberus 1

Cerberus 1, also called DAND4, is a member of the DAN domain family of BMP antagonists that includes DAN (DAND1), Gremlin/Drm (DAND2), PRDC (Protein Related to Dan and Cerberus; DAND3), and COCO/Dante (DAND5). DAN family members contain a cysteine-knot domain that is homologous to that found in other TGF-beta superfamily ligands (1, 2). Mature human Cerberus 1 shares 67% and 68% amino acid (aa) sequence identity with mouse and rat Cerberus 1, respectively. Within the cysteine-knot domain, it shares 24%-37% aa sequence identity with mouse DAN, Gremlin, PRDC, and COCO. Cerberus 1 is a secreted 38 kDa glycoprotein that forms homodimers (3). Cerberus-S, which is generated by proteolysis in Xenopus, is a short version of the molecule and includes the C-terminal cysteine-knot domain (4). At the onset of gastrulation, Cerberus 1 is transiently expressed in anterior endodermal structures in response to Nodal and Shh (3, 5-9). Cerberus 1 binds BMP-4 and Nodal and inhibits their activities. Xenopus Cerberus has also been shown to bind Xenopus Wnt8. These inhibitory functions of Cerberus favor mesodermal development in the anterior region of the gastrula and suppresses posterior mesodermal differentiation (3, 4, 6, 8, 10-12). In chick and Xenopus, Cerberus 1 also regulates, but is not required for embryonic left-right polarization, neurulation, and head and heart induction (4-8, 13).

References

  1. Avsian-Kretchmer, O. and A.J.W. Hsueh (2004) Mol. Endocrinol. 18:1.
  2. Kawano, Y. and R. Kypta (2003) J. Cell Sci. 116:2627.
  3. Biben, C. et al. (1998) Dev. Biol. 194:135.
  4. Piccolo, S. et al. (1999) Nature 397:707.
  5. Zhu, L. et al. (1999) Curr. Biol. 9:931.
  6. Tavares, A.T. et al. (2007) Development 134:2051.
  7. Foley, A.C. et al. (2007) Dev. Biol. 303:57.
  8. Bouwmeester, T. et al. (1996) Nature 382:595.
  9. Bertocchini, F. and C.D. Stern (2002) Dev. Cell 3:735.
  10. Glinka, A. et al. (1997) Nature 389:517.
  11. Perea-Gomez, A. et al. (2002) Dev. Cell 3:745.
  12. Takaoka, K. et al. (2007) Curr. Opin. Genet. Dev. 17:344
  13. Simpson, E.H. et al. (1999) Dev. Biol. 213:202.

Alternate Names

CER1, DAND4

Entrez Gene IDs

9350 (Human); 12622 (Mouse)

Gene Symbol

CER1

UniProt

O95813

Additional Cerberus 1 Products

Product Documents for Recombinant Human Cerberus 1 Protein, CF

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Product Specific Notices for Recombinant Human Cerberus 1 Protein, CF

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