Recombinant Human Chitotriosidase/CHIT1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3559-GH

R&D Systems, part of Bio-Techne
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3559-GH-010
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Key Product Details

Source

NS0

Accession #

NP_003456

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Chitotriosidase/CHIT1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Chitotriosidase/CHIT1 protein
Ala22-Asn466, with a C-terminal 10-His tag

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala22

Predicted Molecular Mass

51 kDa

SDS-PAGE

52 kDa, reducing conditions

Activity

Measured by its ability to cleave 4-Methylumbelliferyl-beta -D-N,N’,N”-triacetylchitotriose (4-MU-TACT).
The specific activity is >1,500 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

3559-GH
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Chitotriosidase/CHIT1

Chitotriosidase encoded by the CHIT1 gene is a member of the chitinase family that is selectively expressed in activated tissue macrophages (1). It is distinct from another member, known as acidic mammalian chitinase (AMC) encoded by the CHIA gene and expressed mainly in the gastrointestinal tract and lung (2). Both CHIA and CHIT1 are secreted as 50 kDa proteins. In contrast to CHIA, CHIT1 is not stable under acidic pHs and can be processed into a C-terminally truncated 39 kDa form (2, 3). CHIT1 is the best biomarker in the monitoring of Gaucher disease among the three most commonly used markers that also include acid phosphatase and angiotensin-converting enzyme (ACE) (4). CHIT1 is also a specific marker of macrophage activation in acute ischemic stroke (5).

References

  1. Aguilera, B. et al. (2003) J. Biol. Chem. 278:40911.
  2. Boot, R.G. et al. (2001) J. Biol. Chem. 276:6770.
  3. Renkema, G.H. et al. (1997) Eur. J. Biochem. 244:279.
  4. Vellodi, A. et al. (2005) J. Inherit. Metab. Dis. 28:585.
  5. Sotgiu, S. et al. (2005) Eur. Neurol. 54:149.

Alternate Names

CHI3, CHIT1

Entrez Gene IDs

1118 (Human)

Gene Symbol

CHIT1

UniProt

NP_003456

Additional Chitotriosidase/CHIT1 Products

Product Documents for Recombinant Human Chitotriosidase/CHIT1 Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Chitotriosidase/CHIT1 Protein, CF

For research use only

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