Recombinant Human Choline Kinase beta Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 8184-CK

R&D Systems, part of Bio-Techne
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8184-CK-050
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Key Product Details

Source

E. coli

Accession #

Q9Y259

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Choline Kinase beta Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human Choline Kinase beta protein
Arg35-Ser395, with an N-terminal Met and a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

43 kDa

SDS-PAGE

40-43 kDa, reducing conditions

Activity

Measured by its ability to phosphorylate choline chloride.
The specific activity is >250 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

8184-CK
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl, TCEP and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: Choline Kinase beta

Choline kinase catalyzes the first reaction in the choline pathway for the biosynthesis of phosphatidylcholine and phosphatidylethanolamine, the major phospholipids in eukaryotic membranes. Phosphatidylcholine is important for a variety of function in eukaryotes such as facilitating the transport of cholesterol through the organism, acting as a precursor for second messengers and as a cofactor for several membrane-related enzymes (1, 2). Choline kinase also plays a vital role in the production of sphingomyelin, another important membrane phospholipid (3). While choline kinase alpha (CHKA) is more active on choline, Choline Kinase beta (CHKB) is more active on ethanolamine (4). These two enzymes can also form active heterodimer and oligomer (5, 6). CHKA is associated with breast, lung and prostate carcinomas (7, 8) and is a promising target for cancer diagnosis and treatment (9). Mice lacks CHKA die early in embryogenesis. In contrast, mice that lack CHKB survive to adulthood, but develop hindlimb muscular dystrophy and forelimb bone deformity (10, 11), suggesting that CHKB is a rate-limiting enzyme in skeletal muscles. The enzymatic activity of recombinant human CHKB is measured using a phosphatase-coupled method (12).

References

  1. Ramírez De Molina, A. et al. (2002) Biochem. Biophys. Res. Commun. 296:580.
  2. Kent, C (1990) Prog Lipid Res. 29:87.
  3. Janardhan, S. et al. (2006). Curr. Med. Chem. 13:1169.
  4. Gallego-Orgega, D. et al. (2009) PLoS ONE 4:E7819.
  5. Aoyama, C. et al. (2004). Prog. Lipid Res. 43:266.
  6. Uchida, T and Yamashita, S (1992). J. Biol. Chem. 267:10156.
  7. Eliyahu, G. et al. (2007) Int. J. Cancer 120:1721.
  8. Ramírez De Molina, A. et al. (2004). Cancer Research 64:6732.
  9. Gallego-Orgega, D. et al. (2011) Adv. Enzyme Regul. 51:183.
  10. Wu, G. and Vance, D.E. (2010) Biochem. Cell Biol. 88:559.
  11. Sher, R.B. et al. (2006) J. Biol. Chem. 281:4938.
  12. Wu, Z.L. (2011) PLoS One 6:e23172.

Alternate Names

CHETK, CHKL, ChoKB, CK, CKEKB, EK, EKB, Ethanolamine Kinase beta, MDCMC

Entrez Gene IDs

1120 (Human); 12651 (Mouse); 29367 (Rat)

Gene Symbol

CHKB

UniProt

Q9Y259

Additional Choline Kinase beta Products

Product Documents for Recombinant Human Choline Kinase beta Protein, CF

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Product Specific Notices for Recombinant Human Choline Kinase beta Protein, CF

For research use only

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