Recombinant Human Chymotrypsin C/CTRC Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 6907-SE

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
6907-SE-010
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

CHO

Accession #

Q99895

Conjugate

Unconjugated

Applications

Enzyme Activity

View all Chymotrypsin C/CTRC Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Chymotrypsin C/CTRC protein
Met1-Leu268, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys17

Predicted Molecular Mass

28.8 kDa

SDS-PAGE

33-40 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, SUC-Ala-Ala-Pro-Phe-AMC.
The specific activity is >400 pmol/min/μg, as measured under the described conditions.

Formulation, Preparation and Storage

6907-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: Chymotrypsin C/CTRC

Chymotrypsin C (CTRC), also known as caldecrin, is a serine protease and a member of the peptidase S1 family. The CTRC gene encodes a serum calcium‑decreasing factor that has chymotrypsin-like protease activity (1). CTRC has broad substrate specificity, but prefers to cleave on the carboxyl side of hydrophobic residues. The enzyme is expressed primarily in the pancreas, and is secreted into the digestive tract. CTRC is a key regulator of activation and degradation of cationic trypsinogen (2, 3). The inappropriate activation of cationic trypsinogen can contribute to the development of pancreatitis (2, 3). CTRC inhibits osteoclast differentiation by suppression of NFATc1 function by a mechanism independent of its proteolytic activity (4).

References

  1. Tomomura, A. et al. (1995) J. Biol. Chem. 270:30315.
  2. Szmola, R. and M. Sahin-Toth (2007) Proc. Natl. Acad. Sci. USA 104:11227.
  3. Nemoda, Z. and M. Sahin-Toth (2006) J. Biol. Chem. 281:11879.
  4. Hasegawa, H. et al. (2010) J. Biol. Chem. 285:25448.

Alternate Names

Caldecrin, CLCR, CTRC, ELA4, Elastase IV

Entrez Gene IDs

11330 (Human); 76701 (Mouse)

Gene Symbol

CTRC

UniProt

Q99895

Additional Chymotrypsin C/CTRC Products

Product Documents for Recombinant Human Chymotrypsin C/CTRC Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human Chymotrypsin C/CTRC Protein, CF

Coomassie is a registered trademark of Imperial Chemical Industries Ltd.

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.