Recombinant Human CLEC14A Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9664-CL

R&D Systems, part of Bio-Techne
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9664-CL-050
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Key Product Details

Source

HEK293

Accession #

Q86T13

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all CLEC14A Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CLEC14A protein
Human CLEC14A
(Glu22-Ala397)
Accession # Q86T13		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu22

Predicted Molecular Mass

66 kDa

SDS-PAGE

94-108 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human CLEC14A Fc Chimera is coated at 1 µg/mL, 100 μL/well, Recombinant Human VEGF R3/Flt‑4 Fc Chimera (Catalog # 349-F4) binds with an ED50 of 0.8-4.8 μg/mL.

Formulation, Preparation and Storage

9664-CL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CLEC14A

C-type lectin domain family 14 member A (CLEC14A), also known as epidermal growth factor receptor 5 (EGFR-5), is an approximately 52-kDa member of the C-type lectin domain family that plays a role in angiogenic functions including cell-cell adhesion, endothelial cell migration, and tube formation (1, 2). This tumor endothelial marker is a type I transmembrane protein whose extracellular domain (ECD) domain consists of a 141 amino acid (aa) C-type lectin domain, a 43 aa EGF-like domain, and a sushi-like domain (3). Within the ECD, human CLEC14A shares 67% and 68% sequence identity with mouse and rat CLEC14A, respectively.In endothelial cells, CLEC14A forms a complex with VEGFR-3. Failure in the formation of this complex has been observed to result in the enhanced expression and activity of VEGFR-2 and the downstream signaling (4). By its interaction with VEGFR-3, CLECA4 appears to regulate developmental angiogenesis, lymphangiogenesis, and pathological angiogensis (4). CLEC14A acts in vascular homeostasis by fine-tuning VEGF R2 and VEGF R3 signaling in ECs, suggesting its relevance in the pathogenesis of angiogenesis-related human disorders (4).

References

  1. Jang, J. et al. (2017) Sci. Rep. 7:10666.
  2. Mura, M. et al. (2012) Oncogene. 31:293.
  3. Zelensky, A. and Gready, J. (2005) FEBS. J. 272:6179.
  4. Lee, S. et al. (2017) J. Clin. Invest. 127:457.

Long Name

C-type Lectin Domain Family 14, Member A

Alternate Names

CEG1, EGF R5

Entrez Gene IDs

161198 (Human); 66864 (Mouse); 314148 (Rat)

Gene Symbol

CLEC14A

UniProt

Q86T13

Additional CLEC14A Products

Product Documents for Recombinant Human CLEC14A Fc Chimera Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human CLEC14A Fc Chimera Protein, CF

For research use only

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