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Recombinant Human Clusterin Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 2937-HS

R&D Systems, part of Bio-Techne
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2937-HS-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked heterodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Clusterin protein
Asp23-Arg227 (beta) & Ser228-Glu449 (alpha) with a C-terminal 6-His tag

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser228 ( alpha chain) & Asp23 ( beta chain)

Predicted Molecular Mass

26.7 kDa ( alpha chain) and 24.2 kDa ( beta chain)

SDS-PAGE

40 kDa and 39 kDa, under reducing conditions

Activity

Measured by its ability to induce clustering of Caki-2 human clear cell carcinoma epithelial cells. Schwochau, G. et al. (1998) Kidney Int. 53:1647.

Reviewed Applications

Read 1 review rated 4 using 2937-HS in the following applications:

Formulation, Preparation and Storage

2937-HS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Clusterin

Clusterin, also known as Apolipoprotein J, Sulfated Glycoprotein 2 (SGP-2), TRPM-2, and SP-40,40, is a secreted multifunctional protein that was named for its ability to induce cellular clustering. It binds a wide range of molecules and may function as a chaperone of misfolded extracellular proteins. It also participates in the control of cell proliferation, apoptosis, and carcinogenesis (1, 2). Clusterin is predominantly expressed in adult testis, ovary, adrenal gland, liver, heart, and brain and in many epithelial tissues during embryonic development (3). Human Clusterin is synthesized as a precursor that contains two coiled coil domains, three nuclear localization signals (NLS), and one heparin binding domain (4-6). Intracellular cleavages of the precursor remove the signal peptide and generate comparably sized alpha and beta chains which are secreted as an 80 kDa N-glycosylated disulfide-linked heterodimer (7, 8). Mature human Clusterin shares 77% amino acid sequence identity with mouse and rat Clusterin. High μg/mL concentrations of Clusterin circulate predominantly as a component of high density lipoprotein particles, and these are internalized and degraded through interactions with LRP-2/Megalin (9, 10). In human, an alternately spliced 50 kDa isoform of Clusterin (nCLU) lacks the signal peptide and remains intracellular (5,  11). This molecule is neither glycosylated nor cleaved into alpha and beta chains (11). In the cytoplasm, nCLU destabilizes the actin cytoskeleton and inhibits NF kappaB activation (12, 13). Cellular exposure to ionizing radiation promotes the translocation of nCLU to the nucleus where it interacts with Ku70 and promotes apoptosis (5, 11). This function contrasts with the cytoprotective effect of secreted Clusterin (14). During colon cancer tumor progression there is a down‑regulation of the intracellular form and an up‑regulation of the glycosylated secreted form (11).

References

  1. Carver, J.A. et al. (2003) IUBMB Life 55:661.
  2. Shannan, B. et al. (2006) Cell Death Differ. 13:12.
  3. French, L.E. et al. (1993) J. Cell Biol. 122:1119.
  4. Kirszbaum, L. et al. (1989) EMBO J. 8:711.
  5. Leskov, K.S. et al. (2003) J. Biol. Chem. 278:11590.
  6. Pankhurst, G.J. et al. (1998) Biochemistry 37:4823.
  7. Burkey, B.F. et al. (1991) J. Lipid. Res. 32:1039.
  8. de Silva, H.V. et al. (1990) J. Biol. Chem. 265:14292.
  9. Jenne, D.E. et al. (1991) J. Biol. Chem. 266:11030.
  10. Kounnas, M.Z. et al. (1995) J. Biol. Chem. 270:13070.
  11. Pucci, S. et al. (2004) Oncogene 23:2298.
  12. Moretti, R. M. et al. (2007) Cancer Res. 67:10325.
  13. Santilli, G. et al. (2003) J. Biol. Chem. 278:38214.
  14. Trougakos, I.P. et al. (2004) Cancer Res. 64:1834.

Alternate Names

APOJ, CLI, CLU, SGP-2, SP-40, TRPM-2

Entrez Gene IDs

1191 (Human); 12759 (Mouse)

Gene Symbol

CLU

UniProt

Additional Clusterin Products

Product Documents for Recombinant Human Clusterin Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Human Clusterin Protein, CF

For research use only

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