Recombinant Human CNTF Protein Best Seller
R&D Systems, part of Bio-Techne | Catalog # 257-NT
Key Product Details
Source
E. coli
Accession #
Structure / Form
This recombinant protein is prone to proteolytic degradation at the C-terminus, resulting in proteins with molecular masses of 22.2-22.8 kDa.
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
E. coli-derived human CNTF protein
Ala2-Met200
Ala2-Met200
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala2
Predicted Molecular Mass
22.8 kDa
SDS-PAGE
23-26 kDa, reducing conditions
Activity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323.
The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) .
The ED50 for this effect is 0.03-0.18 μg/mL.
The proliferative effect can be enhanced up to 100-fold by the addition of Recombinant Human CNTF R alpha (Catalog # 303-CR) .
Scientific Data Images for Recombinant Human CNTF Protein
Recombinant Human CNTF Protein Bioactivity
Recombinant Human CNTF (Catalog # 257‑NT) stimulates cell proliferation of the TF-1 human erythroleukemic cell line. The ED50 for this effect is 0.03-0.18 μg/mL.
Recombinant Human CNTF Protein SDS-PAGE
1 μg/lane of Recombinant Human CNTF was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a band at 26 kDa.Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 257-NT
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 257-NT/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris, NaCl, TCEP, EDTA, CHAPS and Trehalose. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: CNTF
Ciliary neurotrophic factor (CNTF) is a polypeptide initially purified from chick embryo ocular tissue and identified as a trophic factor for embryonic chick ciliary parasympathetic neurons in culture. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons. CNTF has also been shown to prevent the degeneration of motor axons after axotomy.
The gene for human CNTF has been localized to the proximal region of the long arm of chromosome 11. The cDNA for human CNTF encodes a 200 amino acid residue polypeptide that lacks a signal sequence. CNTF is highly conserved across species and exhibits cross-species activities. Human and rat CNTF share approximately 83% homology in their protein sequence. CNTF is structurally related to IL-6, IL-11, LIF and OSM. All of these four helix bundle cytokines share gp130 as a signal-transducing subunit in their receptor complexes.
Long Name
Ciliary Neurotrophic Factor
Alternate Names
ciliary neurotrophic factor, HCNTF
Gene Symbol
CNTF
UniProt
Additional CNTF Products
Product Documents for Recombinant Human CNTF Protein
Product Specific Notices for Recombinant Human CNTF Protein
For research use only
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