Recombinant Human Coagulation Factor Xa Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1063-SE
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Active form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Coagulation Factor X protein
Leu24-Lys488 with a C-terminal 10-His tag
Proform Factor X was expressed, purified, activated and further purified to yield Factor Xa.
Leu24-Lys488 with a C-terminal 10-His tag
Proform Factor X was expressed, purified, activated and further purified to yield Factor Xa.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Tyr84, Phe124 & Ile235
Predicted Molecular Mass
12 kDa & 30 kDa
SDS-PAGE
13-14 kDa and 33-36 kDa, reducing conditions
Activity
Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >700 pmol/min/µg, as measured under the described conditions.
The specific activity is >700 pmol/min/µg, as measured under the described conditions.
Reviewed Applications
Read 1 review rated 5 using 1063-SE in the following applications:
Formulation, Preparation and Storage
1063-SE
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES, NaCl and CaCl2. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl and 5 mM CaCl2, pH 6.0. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Coagulation Factor X
As the only known physiological activator of thrombin, Factor X is a vitamin K-dependent plasma protease that plays a pivotal role in blood coagulation. Human Factor X is initially synthesized in the liver as a single-chain precursor of 488 amino acid residues with a signal peptide and a pro region (residues 1‑40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41‑179) and heavy (residues 235‑488) chains linked by a disulfide bond. The light chain contains a Gla and two EGF-like domains and the heavy chain corresponds to the serine protease domain. The full-length human Factor X was expressed and the pro enzyme was purified and activated. The active protease (rhXa) was further purified and analyzed for its activity towards either peptides or proteins containing a Xa cleavage site. In addition to the activity described in the Activity Assay Protocol, rhFX also be used to cleave fusion proteins containing a Factor Xa cleavage site. The conditions for the optimal cleavage of a particular fusion protein, such as the molar ratio between rhFX and the protein and time and temperature of incubation, are protein-dependent and need to be individually determined.
Alternate Names
Cf10, F10, FX, FXa, Prothrombinase, Stuart Factor
Gene Symbol
F10
UniProt
Additional Coagulation Factor X Products
Product Documents for Recombinant Human Coagulation Factor Xa Protein, CF
Product Specific Notices for Recombinant Human Coagulation Factor Xa Protein, CF
For research use only
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