Recombinant Human Common gamma Chain Protein
R&D Systems, part of Bio-Techne | Catalog # 384-RG
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Common gamma Chain/IL-2 R gamma protein
Leu23-Asn254, with a C-terminal 6-His tag
Leu23-Asn254, with a C-terminal 6-His tag
Purity
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu23
Predicted Molecular Mass
28.2 kDa
SDS-PAGE
40 kDa, reducing conditions
Activity
Measured by its ability to inhibit the IL-2-dependent proliferation of MO7e human megakaryocytic leukemic cells.
The ED50 for this effect is typically 0.6-3.6 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.
The ED50 for this effect is typically 0.6-3.6 µg/mL in the presence of 30 µg/mL of soluble IL-2 R beta and 10 ng/mL of recombinant human IL-2.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 384-RG
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 384-RG/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 500 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Common gamma Chain/IL-2 R gamma
The gamma chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 R gamma is a 369 amino acid residue protein consisting of a 22 residue signal sequence, a 232 residue extracellular domain, a 29 residue transmembrane domain and an 86 residue cytoplasmic domain. Although IL-2 R gamma by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the gamma chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. It has been proposed that IL-2 R gamma be designated the common gamma chain ( gammac). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has now been mapped to the IL-2 R gamma gene.
References
- Minami, Y. et al. (1993) Annu. Rev. Immunol. 11:245.
- Noguchi, M. et al. (1993) Science 262:1877.
Long Name
IL-2 Receptor gamma
Alternate Names
CD132, IL-2 R gamma, IL2R gamma, IL2RG
Gene Symbol
IL2RG
UniProt
Additional Common gamma Chain/IL-2 R gamma Products
Product Documents for Recombinant Human Common gamma Chain Protein
Product Specific Notices for Recombinant Human Common gamma Chain Protein
For research use only
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