Recombinant Human Complement Component C2 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1936-SE
Key Product Details
Source
NS0
Accession #
Structure / Form
Pro form
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Complement Component C2 protein
Ala21-Leu752, with a C-terminal 6-His tag
Ala21-Leu752, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala21
Predicted Molecular Mass
82 kDa
SDS-PAGE
103 kDa, reducing conditions
Activity
Measured by its ability to cleave a colorimetric peptide substrate, N-carbobenzyloxy-Gly-Arg-ThioBenzyl ester (Z-GR-SBzl), in the presence of 5,5’Dithio-bis (2-nitrobenzoic acid) (DTNB). Edwards, K.M. et al. (1999) J. Biol. Chem. 274:30468.
The specific activity is >35 pmol/min/µg, as measured under the described conditions.
The specific activity is >35 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
1936-SE
| Formulation | Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Complement Component C2
The classical complement pathway plays a major role in innate immunity against infection. This pathway is triggered by C1, a multimolecular complex composed of the recognition protein C1q and two serine proteases, C1r and C1s (1). After activation by C1, the single-chain form of C2 (amino acid residues 21‑752) becomes two chains, which are referred to as C2A and C2B. C2A (residues 244‑752) consists of a vWF domain (residues 254‑452) and a serine protease domain (residues 466‑752). C2B (residues 21‑243) contains 3 Sushi (SCR) domains. C2A, then combines with complement factor 4B to generate the C3 or C5 convertase. The full‑length of human C2 was expressed, and the purified protein corresponded to the single-chain form with the peptidase activity as described in the Activity Assay Protocol.
References
- Arlaud, G.J. et al. (2002) Biochem. Soc. Trans. 30:1001.
Alternate Names
C2
Gene Symbol
C2
UniProt
Additional Complement Component C2 Products
Product Documents for Recombinant Human Complement Component C2 Protein, CF
Product Specific Notices for Recombinant Human Complement Component C2 Protein, CF
For research use only
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