Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI11533

R&D Systems, part of Bio-Techne
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AVI11533-050
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Key Product Details

Source

CHO

Accession #

Q9NQ25.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all CRACC/SLAMF7 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human CRACC/SLAMF7 protein
Ser23-Met226, With a C-terminal 6-His tag & Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser 23

Predicted Molecular Mass

25 kDa

SDS-PAGE

44-52 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human CRACC/SLAMF7 His-tag (Catalog # AVI11533) binds Human CRACC/SLAMF7 Antibody (Catalog # MAB19061) with an ED50 of 5.00-60.0 ng/mL.

Scientific Data Images for Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF

Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein Binding Activity.

Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein (Catalog # AVI11533) binds Human CRACC/SLAMF7 Antibody (MAB19061) with an ED50 of 5.00-60.0 ng/mL.

Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human CRACC/SLAMF7 His-tag Protein (Catalog # AVI11533) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 44-52 kDa, under reducing conditions.

Formulation, Preparation and Storage

AVI11533
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.  
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CRACC/SLAMF7

CD2-like receptor activating cytotoxic cells (CRACC), also known as CS1, novel Ly9, SLAMF7, and CD319, is a type I transmembrane glycoprotein in the SLAM subgroup of the CD2 family (1). Mature human CRACC consists of an extracellular domain (ECD) with one Ig-like V-set domain and one Ig-like C2-set domain, a transmembrane segment, and a cytoplasmic domain with one immunoreceptor tyrosine-based switch motif (ITSM) (2, 3). Within the ECD, human CRACC shares 54% and 52% amino acid sequence identity with mouse and rat CRACC, respectively. There are seven known isoforms of CRACC which are distinguished by deletions and/or substitutions in both their ECD and cytoplasmic domains. CRACC is expressed on the surface of NK cells, CD8+ T cells, activated B cells, and mature dendritic cells (4, 5). Its homophilic interaction induces NK, CTL, and B cell activation (4-7). In human NK cells, activated CRACC transmits signals following association with the adaptor protein EAT-2 (8).Our Avi-tag Biotinylated Recombinant Human CRACC/SLAMF7 features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Veillette, A. (2006) Immunol. Rev. 214:22.
  2. Tovar, V. et al. (2002) Immunogenetics 54:394.
  3. Murphy, J.J. et al. (2002) Biochem. J. 361:431.
  4. Bouchon, A. et al. (2001) J. Immunol. 167:5517.
  5. Lee, J.K. et al. (2007) J. Immunol. 179:4672.
  6. Kumaresan, P.R. et al. (2002) Mol. Immunol. 39:1.
  7. Stark, S. and C. Watzl (2006) Int. Immunol. 18:241.
  8. Tassi, H. and M. Colonna (2005) J. Immunol. 175:7996.

Alternate Names

CD319, SLAMF7

Entrez Gene IDs

57823 (Human); 75345 (Mouse); 102133710 (Cynomolgus Monkey)

Gene Symbol

SLAMF7

UniProt

Q9NQ25.1

Additional CRACC/SLAMF7 Products

Product Documents for Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CRACC/SLAMF7 His-tag Avi-tag Protein, CF

For research use only

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