Recombinant Human CTGF/CCN2 Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 9190-CC

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9190-CC-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q5M8T4

Conjugate

Unconjugated

Applications

Bioactivity

View all CTGF/CCN2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CTGF/CCN2 protein
Gln27-Ala349

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln27 inferred from enzymatic pyroglutamate treatment revealing Asn28

Predicted Molecular Mass

35 kDa

SDS-PAGE

35-45 kDa, reducing conditions

Activity

Measured by its ability to mediate Balb/3T3 mouse embryonic fibroblast cell adhesion. Ball, D.K. et al. (2003) Reproduction 125:271.
The ED50 for this effect is1.5-7.5 μg/mL.

Reviewed Applications

Read 1 review rated 5 using 9190-CC in the following applications:

Scientific Data Images for Recombinant Human CTGF/CCN2 Protein, CF

Recombinant Human CTGF/CCN2 Protein Bioactivity

Recombinant Human CTGF/CCN2 Protein Bioactivity

Immobilized Recombinant Human CTGF (Catalog # 9190-CC) supports the adhesion of Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 1.5-7.5 μg/mL.

Formulation, Preparation and Storage

9190-CC
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTGF/CCN2

Connective Tissue Growth Factor (CTGF), also known as CCN2, is a 36-38 kDa member of the CCN (CYR61/CTGF/NOV) family of secreted matricellular proteins (1). Like other CCN proteins, mature human CTGF consists of IGF-binding protein domain, a vWF-C domain, a TSP-1 domain, and a cysteine knot heparin-binding domain (2). Human CTGF shares 94% amino acid (aa) sequence identity with mouse and rat CTGF. Alternative splicing generates an additional isoform with a 27 aa deletion within the TSP-1 domain. CTGF promotes cell adhesion through interactions with a range of cell surface molecules including heparan sulfate proteoglycans (HSPG), LRPAP, and Integrins  alphaM, alphaV beta3, alpha6 beta1, alphaIIb beta3 (3-7). It also binds to and regulates signaling through the Wnt receptors LRP6 and Frizzled-8 and the NGF receptors TrkA and NGF R (8, 9). In addition, CTGF binds directly to BMP-4, TGF-beta 1, TGF-beta 2, and VEGF 165 (10, 11). It blocks BMP-4 and VEGF induced responses but enhances TGF-beta induced responses (10-13). Within VEGF complexes, CTGF can be degraded by a variety of proteases, resulting in restoration of angiogenic activity (11). CTGF promotes fibroblast differentiation from mesenchymal stem cells and their production of type I Collagen and Tenascin C (5, 14). It promotes chondrocyte proliferation and cartilage matrix synthesis (15-17). CTGF is expressed in vascular mural and endothelial cells (EC) during development and promotes pericyte-EC association and angiogenesis (2, 17, 18, 19). It is expressed in the cerebral cortex and olfactory bulb and plays an important role in nervous system development (8, 12).

References

  1. Hall-Glenn, F. and K.M. Lyons (2011) Cell. Mol. Life Sci. 68:3209.
  2. Bradham, D.M. et al. (1991) J. Cell Biol. 114:1285.
  3. Gao, R. and D.R. Brigstock (2004) J. Biol. Chem. 279:8848.
  4. Schober, J.M. et al. (2002) Blood 99:4457.
  5. Heng, E.C.K. et al. (2006) J. Cell Biochem. 98:409.
  6. Jedsadayanmata, A. et al. (1999) J. Biol. Chem. 274:24321.
  7. Gao, R. et al. (2003) Hepatol. Res. 27:214.
  8. Mercurio, S. et al. (2004) Development 131:2137.
  9. Wahab, N.A. et al. (2005) J. Am. Soc. Nephrol. 16:340.
  10. Abreu, J.G. et al. (2002) Nat. Cell Biol. 4:599.
  11. Hashimoto, G. et al. (2002) J. Biol. Chem. 277:36288.
  12. Khodosevich, K. et al. (2013) Neuron 79:1136.
  13. Shi-Wen, X. et al. (2006) J. Biol. Chem. 281:10715.
  14. Lee, C.H. et al. (2010) J. Clin. Invest. 120:3340.
  15. Canalis, E. et al. (2010) Endocrinology 151:3490.
  16. Nakanishi, T. et al. (2000) Endocrinology 141:264.
  17. Ivkovic, S. et al. (2003) Development 130:2779.
  18. Hall-Glenn, F. et al. (2012) PLoS One 7:e30562.
  19. Shimo, T. et al. (1999) J. Biochem. 126:137.

Long Name

Connective Tissue Growth Factor

Alternate Names

CCN2, CTGRP, Fisp12, HCS24, IGFBP-8, NOV2

Entrez Gene IDs

1490 (Human); 14219 (Mouse); 64032 (Rat)

Gene Symbol

CCN2

UniProt

Q5M8T4

Additional CTGF/CCN2 Products

Product Documents for Recombinant Human CTGF/CCN2 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CTGF/CCN2 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.