Recombinant Human CTRP3/C1qTNF3/CORS26 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9398-TN/CF

R&D Systems, part of Bio-Techne
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9398-TN-025/CF
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Key Product Details

Source

NS0

Accession #

Q9BXJ4-1

Structure / Form

Disulfide-linked homo-oligomer

Conjugate

Unconjugated

Applications

Bioactivity

View all CTRP3/C1qTNF3/CORS26 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CTRP3/C1qTNF3/CORS26 protein
Gln23-Lys246

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln23 inferred from enzymetic pyroglutamate treatment revealing Asp24

Predicted Molecular Mass

24 kDa

SDS-PAGE

25-37 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinan Human CTRP3/C1qTNF3/CORS26 is immobilized at 4 µg/mL (100 µL/well), the concentration of Recombinant Human CD36/SR-B3 Fc Chimera (Catalog # 1955-CD) that produces 50% of the optimal binding response is 1.5-7.5 μg/mL.

Scientific Data Images for Recombinant Human CTRP3/C1qTNF3/CORS26 Protein, CF

Recombinant Human CTRP3/C1qTNF3/CORS26 Protein Binding Activity

Recombinant Human CTRP3/C1qTNF3/CORS26 Protein Binding Activity

When Recombinant Human CTRP3/C1qTNF3/CORS26 (Catalog # 9398-TN) is immobilized at 4 µg/mL, Recombinant Human CD36/SR-B3 Fc Chimera (Catalog # 1955-CD) binds with an ED50 of 1.5-7.5 μg/mL.

Formulation, Preparation and Storage

9398-TN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP3/C1qTNF3/CORS26

CORS-26 (collagenous repeat-containing sequence of 26 kDa protein) also known as C1qTNF3 (complement C1q TNF-related protein 3/CTRP3), cartonectin, cartducin, is a 30-32 kDa, secreted member of the C1q and TNF-related (CTRP) superfamily of molecules (1). The mature protein is 224 aa in length. It contains an N-terminal collagen-like domain followed by a C-terminal globular region. Human CORS26 shares 99% aa sequence identity with the mouse CORS26 (2). Like other CTRP members, CORS26 has a trimeric structure and can assemble into hexameric or higher order molecular forms (3). It is expressed by a variety of cells, including adipocytes, cartilage, fibroblasts, monocytes and proliferating chondrocytes (4). The inflammatory effects of LPS, TLR-4 and fatty acids have been shown to be inhibited by CORS26 in adipocytes and monocytes (5). In mouse models, CORS26 has been shown to lower glucose levels and decrease gluconeogenic gene expression (6). Inhibition of 3T3-L1 pre-adipocyte differentiation to adipocytes is associated with CORS26 treatment, demonstrating potential anti-obesity effects (7). Treatment with CORS26 results in the proliferation of skeletal muscle C2C12 cells and inhibition of C2C12 myotube differentiation, mediated by the ERK pathway (8). Due to the variety of functions in metabolism and inflammation, CORS26 is a potential new target of type 2 diabetes treatment.

References

  1. Wong, G.W. et al. (2004) Proc. Natl. Acad. Sci. U.S.A. 101:10302.
  2. Schaffler A. et al. (2003). Biochim Biophys Acta.1630:123.
  3. Suzuki S. et al. (2007) FEBS Lett. 581: 809.
  4. Weigert J. et al. (2005) FEBS Lett. 579: 5565.
  5. Kopp, A. et al. (2010) Endocrinology. 151:5267.
  6. Peterson J. M. et al. (2010) J Biol Chem. 285:39691.
  7. Nishimoto, H. et al. (2017) Cell Bio Int. 41:197.
  8. Otani M. et al. (2015) Mol Cell Biochem. 409:271.

Long Name

C1q and Tumor Necrosis Factor-related Protein 3

Alternate Names

C1qTNF3, Cartducin, CORS26

Entrez Gene IDs

114899 (Human); 81799 (Mouse)

Gene Symbol

C1QTNF3

UniProt

Q9BXJ4-1

Additional CTRP3/C1qTNF3/CORS26 Products

Product Documents for Recombinant Human CTRP3/C1qTNF3/CORS26 Protein, CF

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Product Specific Notices for Recombinant Human CTRP3/C1qTNF3/CORS26 Protein, CF

For research use only

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