Recombinant Human CTRP5/C1qTNF5 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9510-TN

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
9510-TN-050
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

NS0

Accession #

Q9BXJ0

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

View all CTRP5/C1qTNF5 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human CTRP5/C1qTNF5 protein
Ser16-Ala243, with Gln44Arg substitution and a C-terminal 6-His tag

Purity

>85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser16

Predicted Molecular Mass

25 kDa

SDS-PAGE

26-32 kDa, reducing conditions

Activity

Measured by its ability to induce phospho AMPK activation in C2C12 mouse differentiated myocytes.
300 ng/mL of Recombinant Human CTRP5/C1qTNF5 induces phosphorylation of AMPK.

Measured by its binding ability in a functional ELISA.
When Recombinant Human MFRP (Catalog # 1915-MF) is immobilized at 2 μg/mL, 100 μL/well, the concentration of Recombinant Human CTRP5/C1qTNF5 that produces 50% of the optimal binding response is 0.3-1.8 μg/mL.

Scientific Data Images for Recombinant Human CTRP5/C1qTNF5 Protein, CF

Recombinant Human CTRP5/C1qTNF5 Protein Bioactivity

Recombinant Human CTRP5/C1qTNF5 Protein Bioactivity

Recombinant Human CTRP5/C1qTNF5 Induces Phosphorylation of AMPK in C2C12 Differentiated Myocytes. C2C12 differentiated myocytes were treated with Recombinant Human CTRP5/C1qTNF5 (Catalog # 9510-TN) for 1 hour prior to Western blot analysis

Formulation, Preparation and Storage

9510-TN
Formulation Lyophilized from a 0.2 μm filtered solution in HEPES and NaCl.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CTRP5/C1qTNF5

CTRP5, also known as C1qTNF5, belongs to the highly conserved family of Acrp30/Adiponectin paralogs known as C1q and TNF‑related protein family (1). All family members share a modular organization comprising an N‑terminal signal peptide, a short variable region with conserved cysteine residues, a collagenous domain for coiled coil structure, and a C‑terminal globular domain (2, 3). CTRP proteins are predicted to have trimeric structures that can assemble into higher order molecular forms (1). Human and mouse CTRP5 share 94% amino acid sequence identity. CTRP5 is highly expressed in the eye, testis and adipose tissue (4). A mutation (S163R) in C1qTNF5 impairs secretion and is associated with early-onset long anterior zonules (LAZ) and late-onset retinal degeneration (L-ORD) (5). In both mouse and human, the 3' untranslated region of the MFRP transcript contains the complete open reading frame of C1qTNF5, suggesting that these genes are dicistronic. MFRP and C1qTNF5 have been shown to directly interact in the retinal pigment epithelium and are likely functionally related (6). Like other C1qTNF family members, C1qTNF5 shares similarities to adiponectin in structure and function and has been shown to stimulate glucose uptake and increase fatty acid oxidation through activation of AMPK in skeletal muscle (3). Conversely, administration of rhC1qTNF5 was shown to attenuate insulin-induced Akt activation in adipocytes and skeletal muscle (7).

References

  1. Wong, G.W. et al. (2004) Proc. Natl. Acad. Sci. U. S. A. 101:10302.
  2. Thanasupawat, T. et al. (2015) Front. Endocrinol. (Lausanne) 6:127.
  3. Park, S.Y. et al. (2009) J. Biol. Chem. 284:27780.
  4. Schäffler A. and Buechler C. (2012) Trends Endocrinol. Metab. 23:194.
  5. Tu X. and Palczewski K. (2014) J. Struct. Biol. 186:86.
  6. Mandal M.N. et al. (2006) Invest. Ophthalmol. Vis. Sci. 47:5505.
  7. Lei X. et al. (2016) Am. J. Physiol. Endocrinol. Metab. 310:E1036.

Long Name

C1q And Tumor Necrosis Factor Related Protein 5

Alternate Names

C1qTNF5

Entrez Gene IDs

114902 (Human); 235312 (Mouse)

Gene Symbol

C1QTNF5

UniProt

Q9BXJ0

Additional CTRP5/C1qTNF5 Products

Product Documents for Recombinant Human CTRP5/C1qTNF5 Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human CTRP5/C1qTNF5 Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.