Recombinant Human CUL3/RBX2 Neddylated Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-431

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R&D Systems, part of Bio-Techne
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E3-431-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q13618.2 (CUL3), Q9UBF6.1 (RBX2)

Conjugate

Unconjugated

Applications

Bioactivity

View all CUL3/RBX2 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL3/RBX2 Complex protein
Met1 - Ala768 with a N-terminal 10-His tag (CUL3), Met1 - Lys113 (RBX2)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

101 kDa (neddylated CUL3), 13 kDa (RBX2)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation and Storage

E3-431
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL3/RBX2 Complex

Cullin-3 (CUL3) is a core component of multiple BCR (BTB-CUL3-RBX) E3 Ubiquitin ligase complexes that mediate the ubiquitination of several classes of signaling and structural proteins.  In the BCR complex, CUL3 serves as a scaffold that organizes one or more BTB (BR-C, Ttk and Bab, also known as a POZ domain) substrate recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  Substrate specificity of a BCR ligase is determined by the BTB domain protein(s) associated with the ligase, though BTB-independent CUL3 ligase activity has been reported.  In vivo, the E3 ubiquitin ligase of the BCR complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions.  

References

  1. Baek K., et al. (2020) Nature 578: 461.
  2. Choo Y.Y & Hagen T. (2012) PLoS One 7: e41350.
  3. Davidge B., et al. (2019) J. Cell Sci. 132: jcs233049.
  4. Duda D.M., et al. (2012) Mol. Cell 47: 371.
  5. Stogios P.J. et al. (2005) Genome Biol. 6: R82.

Long Name

Cullin 3/Ring Box Protein 2

UniProt

Q13618.2 (CUL3), Q9UBF6.1 (RBX2)

Additional CUL3/RBX2 Complex Products

Product Documents for Recombinant Human CUL3/RBX2 Neddylated Complex Protein, CF

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Product Specific Notices for Recombinant Human CUL3/RBX2 Neddylated Complex Protein, CF

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