Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-651

His10 Neddylated
R&D Systems, part of Bio-Techne
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E3-651-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1), Q96SW2.1 (CRBN)

Conjugate

Unconjugated

Applications

Bioactivity

View all CUL4A/RBX1/DDB1/CRBN Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL4A/RBX1/DDB1/CRBN Complex protein
This complex consists of an N-terminal 10-His tagged, neddylated Cullin-4A , untagged RBX1, untagged DDB1, and untagged CRBN.

Purity

>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

104 kDa (Neddylated CUL4A), 12 kDa (RBX1), 127 kDa (DDB1), 51 kDa (CRBN)

Activity

Typical concentration to support in vitro conjugation reactions will depend on experimental conditions.

Formulation, Preparation and Storage

E3-651
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL4A/RBX1/DDB1/CRBN Complex

Cullin-4A is a core component of multiple cullin-RING type ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cell cycle progression, DNA repair and other processes.  In the DCX complex (DDB1-Cul4-x-box), Cullin-4A serves as a scaffold that organizes the DDB1-X-box recognition subunits with the Rbx1 subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the SCF complex is dependent on the neddylation of the cullin subunit, though neddylation may be dispensable in some in vitro reactions.  

References

  1. Fischer, E.S., et al.  (2014) Nature  512:49.
  2. He, Y.J., et al.  (2006) Genes Dev.  20:2949.
  3. Ito, T., et al.  (2010) Science  327:1345.
  4. Wang, H., et al. (2006) Mol. Cell  22:383.

Long Name

Cullin-4A/RING Box Protein 1/Damage Specific DNA Binding Protein 1/Cereblon Complex

UniProt

Q13619.3 (CUL4A); P62877.1 (RBX1); Q16531.1 (DDB1), Q96SW2.1 (CRBN)

Additional CUL4A/RBX1/DDB1/CRBN Complex Products

Product Documents for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human CUL4A/RBX1/DDB1/CRBN Complex Protein, CF

For research use only

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