Recombinant Human CUL5/RBX2 Neddylated Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-451

His10.
R&D Systems, part of Bio-Techne
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q93034.4 (CUL5); Q9UBF6.1 (RBX2)

Conjugate

Unconjugated

Applications

Bioactivity

View all CUL5/RBX2 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human CUL5/RBX2 Complex protein
Met1 - Ala780 with a N-terminal 10-His tag (CUL5); Met1 - Lys113 (RBX2)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

102 kDa (neddylated CUL5), 13 kDa (RBX2)

Activity

Typical enzyme concentration to support in vitro conjugation will depend on experimental conditions.

Formulation, Preparation and Storage

E3-451
Formulation Supplied as a solution in HEPES, NaCl, Glycerol and DTT.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: CUL5/RBX2 Complex

Cullin-5 (CUL5) is a core component of multiple ECS (Elongin-CUL5-SOCS-box protein) E3 ubiquitin ligase complexes that mediate the ubiquitination of proteins involved in cytokine receptor signal transduction.  In the ECS complex, CUL5 serves as a scaffold that organizes the Elongin B/Elongin C/SOCS (Suppressor of Cytokine Signaling) recognition subunits with the RBX subunit and contributes to catalysis through positioning of the substrate and an E2 ubiquitin-conjugating enzyme.  In vivo, the E3 ubiquitin ligase activity of the ECS complex is dependent on neddylation of the cullin subunit, though neddylation may be dispensable for some in vitro reactions. 

References

  1. Baek, K., et al. (2020) Nature 578: 461.
  2. Duda, D.M., et al. (2012) Mol. Cell 47: 371.
  3. Kamura, T., et al. (2004) Genes Dev. 18: 3055.
  4. Kamura, T., et al. (2001) J. Biol. Chem. 276: 29748.

Long Name

Cullin 5/Ring Box Protein 2

UniProt

Q93034.4 (CUL5); Q9UBF6.1 (RBX2)

Additional CUL5/RBX2 Complex Products

Product Documents for Recombinant Human CUL5/RBX2 Neddylated Complex Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human CUL5/RBX2 Neddylated Complex Protein, CF

For research use only

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