Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI365

Biotinylated
R&D Systems, part of Bio-Techne
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AVI365-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P78423.1

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Binding Activity, Bioactivity

View all CX3CL1/Fractalkine Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human CX3CL1/Fractalkine protein
Human CX3CL1/Fractalkine
(Gln25-Thr338)
Accession # P78423.1		 HHHHHH  Avi-tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln25, inferred from enzymatic pyroglutamate treatment revealing His26

Predicted Molecular Mass

36 kDa

SDS-PAGE

88-103 kDa, under reducing conditions

Activity

Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CX3CR1.
The ED50 for this effect is 20-100 ng/mL.

Measured by its binding ability in a functional ELISA.
When Recombinant Human CX3CL1/Fractalkine monoclonal antibody (Catalog # MAB3651) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with ED50 of 0.15-0.9 μg/mL.

Scientific Data Images for Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein Binding Activity

Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein Binding Activity

When Human CX3CL1/Fractalkine MAb MAB3651) is immobilized at 1 µg/mL (100 µL/well), Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag binds with an ED50 of 0.15-0.9 µg/mL.
Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein SDS-PAGE

Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human CX3CL1/Fractalkine His-tag Avi-tag Protein (Catalog # AVI365) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 88-103;kDa.

Formulation, Preparation and Storage

AVI365
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CX3CL1/Fractalkine

Fractalkine, also known as CX3C motif chemokine 1, CX3CL1, neurotactin and small-inducible cytokine D1, is the only member of the CX3C subfamily of the chemokine superfamily (1). Mature human Fractalkine consists of an N-terminal chemokine domain with a CX3C motif and a mucin-like stalk region in the extracellular domain (ECD), a transmembrane segment and a short cytoplasmic domain (1, 2). The soluble form of Fractalkine is generated via ADAM10 and ADAM17 cleavage (1). Within the ECD, human Fractalkine shares 59% amino acid sequence identity with both mouse and rat Fractalkine. Fractalkine exists as both a membrane-bound adhesion molecule and as a soluble proinflammatory chemoattractant and anti-inflammatory neuroprotective agent (1-3). The expression of CX3CL1 is higher in spinal metastases from kidney cancer (4). The expression of CX3CL1 was also reported to be up-regulated in endothelial cells and microglia by inflammatory signals. Membrane-bound CX3CL1 has been shown to promote adhesion of leukocytes. The soluble chemokine domain of human CX3CL1 was reported to be chemotactic for T cells and monocytes while the soluble chemokine domain of mouse CX3CL1 was reported to chemoattract neutrophils and T-lymphocytes but not monocytes (5). Most of the functions of CX3CL1 are exerted through the CX#CL1/CX3CR1 axis which has the therapeutic prospect (5, 6). Our Avi-tag Biotinylated Recombinant Fractalkine features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Poniatowski, L. et al. (2017) Mol. Neurobiol. 54:2167.
  2. Desforges, N. et al. (2012) Int. J. Alzheimers Dis. 2012:345472.
  3. Nanki, T. et al. (2016) Mod. Rheumatol. 27:392.
  4. Liu, W. et al. (2016) Arch Immunol. Ther. Exp. (Warsz) 64:371.
  5. Zlotnik, A. Yoshie, O. (2012) Immunity 36:705.
  6. Quan, Z. et al. (2017) Current Gene Therapy 17:442.

Alternate Names

FKN, Fractalkine, Neurotactin

Entrez Gene IDs

6376 (Human); 20312 (Mouse); 89808 (Rat); 102117496 (Cynomolgus Monkey)

Gene Symbol

CX3CL1

UniProt

P78423.1

Additional CX3CL1/Fractalkine Products

Product Documents for Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

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Product Specific Notices for Recombinant Human CX3CL1/Fractalkine His Avi-tag Protein, CF

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