Recombinant Human CXCL17/VCC-1 Protein

R&D Systems, part of Bio-Techne | Catalog # 4207-DM

R&D Systems, part of Bio-Techne
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Carrier Free
4207-DM-025/CF
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With Carrier
4207-DM-025
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Key Product Details

Source

E. coli

Accession #

NP_940879

Conjugate

Unconjugated

Applications

Bioactivity

View all CXCL17/VCC-1 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived human CXCL17/VCC-1 protein
Leu24-Leu119

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu24

Predicted Molecular Mass

11.3 kDa

Activity

Measured by its ability to induce VEGF expression in mouse endothelial cells. Weinstein, E.J. et al. (2006) BBRC 350:74.
The ED50 for this effect is 1-5 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 4207-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 4207-DM/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CXCL17/VCC-1

Dendritic cell and monocyte chemokine-like protein (DMC), also known as VEGF-correlated chemokine-1 (VCC-1) and CXCL17, is a novel secreted molecule that belongs to the intercrine alpha (or CXC) family of chemokines (1, 2). It has no predicted N‑glycosylation sites, so cleavage of a 22 amino acid (aa) signal sequence likely results in a mature human DMC of 97 aa and 11 kDa. DMC is constitutively produced by bronchiolar, mammary, and intestinal epithelium, vascular endothelial cells, and multiple tumor types (1, 2, 3). It induces the chemotaxis of quiescent, but not LPS-activated, peripheral blood monocytes and dendritic cells, and also is noted to specifically bind to these cells (1). DMC expression is increased in endothelial cells when they are induced to form tubes in vitro (2). Transgenic overexpression in NIH3T3 cells causes up‑regulation of proteins such as VEGF and FGF basic, and increases cell growth rate and tumorigenicity (2). DMC and two other chemokines that play roles in angiogenesis, CXCL1/GRO and CXCL8/IL-8, show significantly correlated expression with that of VEGF in primary lung, breast and esophageal tumors (2). DMC is therefore suggested to play a role in tumor angiogenesis. Mature human DMC shares 73%, 71% and 64% amino acid sequence identity with bovine, mouse and rat DMC, respectively.

References

  1. Pisabarro, M. T. et al. (2006) J. Immunol. 176:2069.
  2. Weinstein, E. J. et al. (2006) Biochem. Biophys. Res. Commun. 350:74.
  3. Mu, X. et al. (2009) Acta Biochim. Biophys. Sin 41:632.

Alternate Names

CXCL17, DMC, VCC-1, VCC1

Entrez Gene IDs

284340 (Human); 232983 (Mouse)

Gene Symbol

CXCL17

UniProt

NP_940879

Additional CXCL17/VCC-1 Products

Product Documents for Recombinant Human CXCL17/VCC-1 Protein

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 4207-DM (with carrier)
Download SDS 4207-DM/CF (carrier free)

Product Specific Notices for Recombinant Human CXCL17/VCC-1 Protein

For research use only

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