Recombinant Human Cystatin A Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1407-PI
Key Product Details
Product Specifications
Source
E. coli-derived human Cystatin A protein
Ile2-Phe98, with an N-terminal Met and 6-His tag
Ile2-Phe98, with an N-terminal Met and 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Met
Predicted Molecular Mass
12 kDa
SDS-PAGE
12 kDa, reducing conditions
Activity
Measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC (Catalog # ES009).
The IC50 value is <3.4 nM, as measured under the described conditions.
The IC50 value is <3.4 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1407-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in Tris and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM Tris and 100 mM NaCl, pH 7.5. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin A
Cystatin A, also called stefin A, Cystatin AS or keratolinin, is a member of family 1 of the cystatin superfamily (1, 2). Like Cystatin B, it is an intracellular inhibitor regulating the activities of cysteine proteases of the papain family such as cathepsins B, H and L (3). For example, immunohistochemical analysis of Cystatin A and cathepsin L is a useful indicator for malignancy in human epidermal keratinocytes (4). The ratio of cathepsin B and Cystatin A can be used in the differential diagnosis and treatment of patients with prostate carcinoma (5). The human Cystatin A consists of 98 amino acids (1).
References
- Kartasova, T. et al. (1987) Nucleic Acids Res. 15:5945.
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Jenko, S. et al. (2003) J. Mol. Biol. 326:875.
- Palungwachira, P. et al. (2002) J. Dermatol. 29:573.
- Sinha, A.A. et al. (2002) Cancer 94:3141.
Alternate Names
AREI, CSTA, Stefin A, STF1, STFA
Entrez Gene IDs
1475 (Human)
Gene Symbol
CSTA
UniProt
Additional Cystatin A Products
Product Documents for Recombinant Human Cystatin A Protein, CF
Product Specific Notices for Recombinant Human Cystatin A Protein, CF
For research use only
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