Recombinant Human Cystatin SN Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 1285-PI
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human Cystatin SN protein
Trp21-Ser141, with a C-terminal 10-His tag
Trp21-Ser141, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Trp21
Predicted Molecular Mass
16 kDa
SDS-PAGE
16 kDa, reducing conditions
Activity
Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic peptide substrate Z-LR-AMC (Catalog # ES008).
The IC50 value is <7.0 nM, as measured under the described conditions.
The IC50 value is <7.0 nM, as measured under the described conditions.
Formulation, Preparation and Storage
1285-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile 25 mM MES, 150 mM NaCl, pH 6.5. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Cystatin SN
Cystatin SN is a member of family 2 of the cystatin superfamily (1). Together with cystatins S and SA, it is produced by the salivary gland and secreted largely in the submandibular/sublingual saliva (2). Cystatin SN inhibits members of the papain family including cathepsins B, C, H and L (3).
References
- Abrahamson, M. (1994) Methods Enzymol. 244:685.
- Baron, A.C. et al. (1999) Oral Dis. 5:344.
- Baron, A. et al. (1999) Oral Dis. 5:234.
Alternate Names
CST1
Entrez Gene IDs
1469 (Human)
Gene Symbol
CST1
UniProt
Additional Cystatin SN Products
Product Documents for Recombinant Human Cystatin SN Protein, CF
Product Specific Notices for Recombinant Human Cystatin SN Protein, CF
For research use only
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