Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 142-DC
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Structure / Form
Disulfide-linked homodimer
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DcR3/TNFRSF6B protein
| Human DcR3 (Val 24-His300) Accession # O95407 |
IEGRMD | Human IgG1 (Pro100-Lys330) |
| N-terminus | C-terminus |
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Val24
Predicted Molecular Mass
57 kDa (monomer)
SDS-PAGE
60-65 kDa, reducing conditions
Activity
Measured by its ability to inhibit Fas Ligand-induced apoptosis of Jurkat human acute T cell leukemia cells. Cheng, J. et al. (1994) Science 263:1759.
The ED50 for this effect is 0.03-0.15 µg/mL in the presence of 20 ng/mL rhFas Ligand.
The ED50 for this effect is 0.03-0.15 µg/mL in the presence of 20 ng/mL rhFas Ligand.
Formulation, Preparation and Storage
142-DC
| Formulation | Lyophilized from a 0.2 μm filtered solution in MES and NaCl. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: IAP Inhibitors
Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.
References
- Pitti, R.M. et al. (1998) Nature 396:699.
- Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
- Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.
UniProt
Additional IAP Inhibitors Products
Product Documents for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF
Product Specific Notices for Recombinant Human DcR3/TNFRSF6B Fc Chimera Protein, CF
For research use only
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