Recombinant Human DDB1/CRBN Complex Protein, CF

R&D Systems, part of Bio-Techne | Catalog # E3-500

R&D Systems, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
E3-500-025
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q16531.1, Q96SW2.1

Conjugate

Unconjugated

Applications

Bioactivity

View all DDB1/CRBN Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human DDB1/CRBN Complex protein

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

127 kDa (DDB1), 51 kDa (CRBN)

Activity

Typical concentration to support in vitro applications will depend on experimental conditions.

Formulation, Preparation and Storage

E3-500
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: DDB1/CRBN Complex

Cereblon (CRBN) is the substrate recognition component of DCX (DDB1-CUL4-X-box) E3 Ubiquitin ligase complexes that mediate the ubiquitination of numerous target proteins including the transcriptional regulator MEIS2.  CRBN plays an important role in limb outgrowth, and its function in this process is perturbed by the binding of thalidomide and related compounds.  Binding of CRBN to Cullin-4 is mediated in part by DNA damage-binding protein 1 (DDB1), a component of multiple DCX class ligases.  In addition to its role in limb development, DDB1 is also involved in various DNA damage response mechanisms.  This product consists of an untagged complex of DDB1 and CRBN.

References

  1. Fischer E.S. ,et al. (2014) Nature  512: 49
  2. He Y.J., et al. (2006)  Genes Dev.  20: 2949
  3. Ito T., et al. (2010) Science  327: 1345
  4. Wang H., et al. (2006) Mol. Cell  22: 383

Long Name

Damage-specific DNA Binding Protein 1/Cereblon Complex

Entrez Gene IDs

51185 (Human); 58799 (Mouse); 297498 (Rat)

Gene Symbol

CRBN

UniProt

Q16531.1, Q96SW2.1

Additional DDB1/CRBN Complex Products

Product Documents for Recombinant Human DDB1/CRBN Complex Protein, CF

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Recombinant Human DDB1/CRBN Complex Protein, CF

For research use only

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.