Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 947-DM

R&D Systems, part of Bio-Techne
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947-DM-100
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Key Product Details

Source

NS0

Accession #

CAA81226

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Desmoglein-2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Desmoglein-2 protein
Human Desmoglein-2
(Ala49-Gly608)
Accession # CAA81226		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala49

Predicted Molecular Mass

89 kDa (monomer)

SDS-PAGE

115 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of BUD-8 human fibroblast cells.
When 3 x 104 cells are added to recombinant human Desmoglein‑2 coated plates (10 μg/mL, 100 μL/well), approximately 50%‑80% will adhere after 1 hour at 37°C.  Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

947-DM
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Desmoglein-2

Desmoglein-2 (DSG2) is a 160 kDa transmembrane glycoprotein in the cadherin family of calcium dependent adhesion molecules. It is a major protein component of desmosomal contacts between epithelial cells (1, 2). Mature human Desmoglein-2 consists of a 560 amino acid (aa) extracellular domain (ECD) with four cadherin domains, a 25 aa transmembrane segment, and a 484 aa cytoplasmic domain with six desmoglein repeats (3). Within the ECD, human Desmoglein-2 shares 81% aa sequence identity with mouse and rat Desmoglein-2. A 60 kDa ECD fragment can be proteolytically shed, and this is enhanced by EGF R activation (4‑6). The cytoplasmic domain is cleaved during apoptosis, leaving the transmembrane segment and ECD as a cell-associated fragment (7). Desmoglein-2 is widely expressed in epithelia and is required for maintenance of the epithelial barrier (1, 2, 8). It can be either up- or down-regulated in a variety of carcinomas during tumor progression (9). Desmoglein-2 also functions as a cell adhesion receptor for several adenovirus serotypes (10). This binding disrupts intercellular junctions between epithelial cells and promotes epithelial-mesenchymal transition (EMT) (10). Mutations in Desmoglein-2 cause cardiac dysfunction typical of arrythmogenic right ventricular cardiomyopathy (ARVC) (11).

References

  1. Brooke, M.A. et al. (2012) J. Pathol. 226:158.
  2. Thomason, H.A. et al. (2010) Biochem. J. 429:419.
  3. Schafer, S. et al. (1994) Exp. Cell Res. 211:391.
  4. Bech-Serra, J.J. et al. (2006) Mol. Cell. Biol. 26:5086.
  5. Klessner, J.L. et al. (2009) Mol. Biol. Cell 20:328.
  6. Ramani, V.C. et al. (2008) BMC Cancer 8:373.
  7. Nava, P. et al. (2007) Mol. Biol. Cell 18:4565.
  8. Schlegel, N. et al. (2010) Am. J. Physiol. Gastrointest. Liver Physiol. 298:G774.
  9. Dusek, R.L. and L.D. Attardi (2011) Nat. Rev. Cancer 11:317.
  10. Wang, H. et al. (2011) Nat. Med. 17:96.
  11. Pilichou, K. et al. (2009) J. Exp. Med. 206:1787.

Alternate Names

CDHF5, Desmoglein2, DSG2, HDGC

Entrez Gene IDs

1829 (Human); 13511 (Mouse)

Gene Symbol

DSG2

UniProt

CAA81226

Additional Desmoglein-2 Products

Product Documents for Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human Desmoglein-2 Fc Chimera Protein, CF

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