Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein
R&D Systems, part of Bio-Techne | Catalog # AFR9749
Key Product Details
Learn more about FluorokinesTM Fluorescent-Labeled Proteins
Source
HEK293
Accession #
Structure / Form
Labeled with Alexa Fluor® 647
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Excitation Wavelength: 650 nm
Emission Wavelength: 668 nm
Conjugate
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)
Applications
Bioactivity
Product Specifications
Source
Human embryonic kidney cell, HEK293-derived human DLL3 protein
| Human DLL3 (Ala27-Ala479) Accession # Q9NYJ7.1 |
HP | GGGSGGGSGGGS | 6-His tag |
| N-terminus | C-terminus | ||
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Ala27
Predicted Molecular Mass
49 kDa
SDS-PAGE
52-62 kDa, under reducing conditions.
Activity
Measured
by flow cytometry for its ability to bind Human DLL3 Antibody conjugated
fluorescent beads at 5.00-20.0 µg/mL (100 µL/well).
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
Scientific Data Images for Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein
Detection of Human DLL3 Antibody with Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein by Flow Cytometry.
Fluorescent beads conjugated to Human DLL3 Antibody were stained with (A) Recombinant Human DLL3 His-tag Alexa Fluor® 647 (Catalog # AFR9749, filled histogram) or (B) unstained (open histogram).
Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein SDS-PAGE.
2 μg/lane of Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein (Catalog # AFR9749) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 52-62 kDa.Formulation, Preparation and Storage
AFR9749
| Formulation | Supplied as a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: DLL3
References
- Dunwoodie, S. L. et al. (1997) Development 124:3065.
- Bulman, M. P. et al. (2000) Nat. Genet. 24:438.
- Ladi, E. et al. (2005) J. Cell Biol. 170:983.
- Dunwoodie, S.L. et al. (2002) Development 129:1795.
- Deng S.M. et al. (2017) Biochem. Biophys. Res. Commun. 483:488.
Long Name
Delta-like 3
Alternate Names
Pudgy, SCDO1
Gene Symbol
DLL3
UniProt
Additional DLL3 Products
Product Documents for Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein
Product Specific Notices for Recombinant Human DLL3 His-tag Alexa Fluor® 647 Protein
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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