Recombinant Human DLL3 Protein, CF Best Seller

R&D Systems, part of Bio-Techne | Catalog # 9749-DL

R&D Systems, part of Bio-Techne
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9749-DL-050
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Key Product Details

Source

HEK293

Accession #

Q9NYJ7.1

Conjugate

Unconjugated

View all DLL3 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human DLL3 protein
Human DLL3
(Ala27-Ala479)
Accession # Q9NYJ7.1		 HP GGGSGGGSGGGS HHHHHH
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala27

Predicted Molecular Mass

49 kDa

SDS-PAGE

52-62 kDa, reducing conditions

Scientific Data Images for Recombinant Human DLL3 Protein, CF

Recombinant Human DLL3 Protein SDS-PAGE

Recombinant Human DLL3 Protein SDS-PAGE

2 μg/lane of Recombinant Human DLL3 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 52-62 kDa and 40-55 kDa, respectively.

Formulation, Preparation and Storage

9749-DL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DLL3

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands (1). Mature human DLL3 consists of a 466 amino acid (aa) extracellular domain (ECD) with one DSL domain and six EGF-like repeats, a 21 aa transmembrane segment, and a 105 aa cytoplasmic domain (2). Within the ECD, human DLL3 shares 86% aa sequence identity with mouse and rat DLL3. DLL3 is known as a divergent DSL ligand since it does not function in similar manner as the rest of DSL ligands. It does not activate Notch signaling through trans-activation, but autonomously attenuates signaling induced by other DSL ligands (3). A loss-of-function mutation of DLL3 is linked to axial skeletal defects in the spondylocostal dysplasia, which is linked to abnormal Notch signaling (4). DL-3 promotes proliferation and inhibits apoptosis of cancer cells. The proliferative effect mediated by DLL3 is thought to be due to increased Akt phosphorylation in cancer cells (5).

References

  1. Dunwoodie, S. L. et al. (1997) Development 124:3065.
  2. Bulman, M. P. et al. (2000) Nat. Genet. 24:438.
  3. Ladi, E. et al. (2005) J. Cell Biol. 170:983.
  4. Dunwoodie, S.L. et al. (2002) Development 129:1795.
  5. Deng S.M. et al. (2017) Biochem. Biophys. Res. Commun. 483:488.

Long Name

Delta-like 3

Alternate Names

Pudgy, SCDO1

Entrez Gene IDs

10683 (Human); 13389 (Mouse); 114125 (Rat)

Gene Symbol

DLL3

UniProt

Q9NYJ7.1

Additional DLL3 Products

Product Documents for Recombinant Human DLL3 Protein, CF

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Product Specific Notices for Recombinant Human DLL3 Protein, CF

For research use only

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