Recombinant Human DMP-1 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4129-DM
Key Product Details
Product Specifications
Source
Mouse myeloma cell line, NS0-derived human DMP-1 protein
Leu17-Tyr497 & Asp202-Tyr497, both with a C-terminal 6-His tag & Leu17-Ser201
Leu17-Tyr497 & Asp202-Tyr497, both with a C-terminal 6-His tag & Leu17-Ser201
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Leu17 & Asp202
Predicted Molecular Mass
53.1 kDa, 19.7 kDa and 33.4 kDa
SDS-PAGE
20-90 kDa, reducing conditions
Activity
Measured by its binding ability in a functional ELISA.
Immobilized rhIntegrin alphav beta3 at 2 µg/mL can bind rhDMP-1 with an apparent
KD <20 nM.
Immobilized rhIntegrin alphav beta3 at 2 µg/mL can bind rhDMP-1 with an apparent
KD <20 nM.
Formulation, Preparation and Storage
4129-DM
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution |
Reconstitute at 100 μg/mL in sterile PBS.
|
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: DMP-1
Dentin matrix protein 1 (DMP-1) is a member of the SIBLING family that also includes bone sialoprotein, dentin sialophosphoprotein, MEPE, and osteopontin. These highly phosphorylated integrin-binding proteins are rich in acidic amino acids and function in the formation of calcified bone and tooth matrix (1, 2). The phosphate content, spacing of acidic residues, and calcium-dependent dimerization of DMP-1 contribute to its ability to sequester calcium phosphate clusters and promote hydroxyapatite (HA) crystal formation (3 - 5). Rodent DMP-1 is cleaved by BMP-1 family proteases at a single site which is conserved in human, generating a 37 kDa N-terminal and a 57 kDa C-terminal fragment (6). The N-terminal fragment in rat carries chondroitin sulfate (7). The C-terminal fragment alone can nucleate HA crystals, while crystal growth into a needle-like morphology is inhibited by the N-terminal fragment (3, 4). Crystal maturation is dependent on the presence of type I collagen (4). DMP-1 is required for odontoblast differentiation as well as dentin formation (8). Nonphosphorylated DMP-1 is targeted to the nucleus, where it activates the transcription of odontoblast and osteoblast specific genes (9, 10). Early in osteoblast maturation, nuclear DMP-1 is extensively phosphorylated by casein kinase II, triggering its secretion (9). DMP-1 mutations in humans are associated with hypophosphatemia and FGF23 overexpression (11, 12). DMP-1 induces the activation of proMMP-9 and displaces mature MMP-9 from TIMP1 (13). DMP-1 tethering of MMP-9 to the cell surface via CD44 and integrins alphav beta3 and alphav beta5 promotes tumor cell invasiveness in vitro (14). Full length DMP-1 circulates in human serum in a tight complex with complement factor H (13, 14). When first bound to CD44 or integrin alphav beta3, DMP-1 can anchor factor H to the cell surface and protect the cell from complement-mediated lysis (15). Mature human DMP-1 shares 61% - 67% amino acid sequence identity with bovine, mouse, and rat DMP-1.
References
- Qin, C. et al. (2004) Crit. Rev. Oral Biol. Med. 15:126.
- Hirst, K.L. et al. (1997) Genomics 42:38.
- He, G. et al. (2003) Nat. Mater. 2:552.
- Gajjeraman, S. et al. (2007) J. Biol. Chem. 282:1193.
- He, G. et al. (2005) Biochemistry 44:16140.
- Steiglitz, B.M. et al. (2004) J. Biol. Chem. 279:980.
- Qin, C. et al. (2006 J. Biol. Chem. 281:8034.
- Lu, Y. et al. (2007) Dev. Biol. 303:191.
- Narayanan, K. et al. (2003) J. Biol. Chem. 278:17500.
- Narayanan, K. et al. (2006) J. Biol. Chem. 281:19064.
- Lorenz-Depiereux, B. et al. (2006) Nat. Genet. 38:1248.
- Feng, J.Q. et al. (2006) Nat. Genet. 38:1310.
- Fedarko, N.S. et al. (2004) FASEB J. 18:735.
- Karadag, A. et al. (2005) Cancer Res. 65:11545.
- Jain, A. et al. (2002) J. Biol. Chem. 277:13700.
Long Name
Dentin Matrix Protein 1
Alternate Names
DMP1
Gene Symbol
DMP1
UniProt
Additional DMP-1 Products
Product Documents for Recombinant Human DMP-1 Protein, CF
Product Specific Notices for Recombinant Human DMP-1 Protein, CF
For research use only
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