Recombinant Human Dopa Decarboxylase/DDC Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 3564-DC
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human Dopa Decarboxylase/DDC protein
Met1-Glu480, with a C-terminal 6-His tag
Met1-Glu480, with a C-terminal 6-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
No result obtained
Predicted Molecular Mass
55 kDa
SDS-PAGE
55 kDa, reducing conditions
Activity
Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. Sherald, F. et al. (1973) Anal. Biochem. 56:300.
The specific activity is >1500 pmol/min/µg, as measured under the described conditions.
The specific activity is >1500 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
3564-DC
| Formulation | Supplied as a 0.2 μm filtered solution in MES, NaCl and DTT. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: Dopa Decarboxylase/DDC
Dopa Decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).
References
- Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
- Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.
Alternate Names
AADC, DDC
Entrez Gene IDs
1644 (Human)
Gene Symbol
DDC
UniProt
Additional Dopa Decarboxylase/DDC Products
Product Documents for Recombinant Human Dopa Decarboxylase/DDC Protein, CF
Product Specific Notices for Recombinant Human Dopa Decarboxylase/DDC Protein, CF
For research use only
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