Recombinant Human DSCAM-L1 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3315-DL

R&D Systems, part of Bio-Techne
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3315-DL-050
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Key Product Details

Source

NS0

Accession #

NP_065744

Conjugate

Unconjugated

Applications

Bioactivity

View all DSCAM-L1 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DSCAM-L1 protein
Glu79-Lys1651, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu79

Predicted Molecular Mass

173.4 kDa

SDS-PAGE

180-200 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated recombinant human DSCAM-L1 in a functional ELISA with an apparent KD <30 nM.

Formulation, Preparation and Storage

3315-DL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSCAM-L1

Down syndrome cell adhesion molecule-like protein 1 (DSCAM-L1; also DSCAM2) is a 224 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM-L1 is a DSCAM paralog located on chromosome 11q23. It is synthesized as a 2053 amino acid (aa) precursor that contains an 18 aa signal sequence, a 1573 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 441 aa cytoplasmic tail. The ECD contains ten Ig-like C2-type domains, six fibronectin type-III domains, and 18 potential sites for N-linked glycosylation. A deletion of aa 34 ‑ 244 produces a second isoform. When compared to DSCAM, DSCAM-L1 shows 64% aa identity to the ECD and 45% aa identity to the cytoplasmic domain (1).  Human DSCAM-L1 is 95% aa identical to mouse DSCAM-L1. In the mouse brain, DSCAM-L1 is predominantly expressed in Purkinje cells of the cerebellum, granule cells of the dentate gyrus, and in neurons of the cerebral cortex and olfactory bulb (1). DSCAM-L1 exhibits homophilic binding activity that does not require divalent cations (1). Based on its similarities to DSCAM, it is postulated that DSCAM-L1 is involved in the formation and maintenance of neural networks (1). Because of its chromosomal location, DSCAM-L1 is an ideal candidate for neuronal disorders such as Gilles de la Tourette and Jacobsen syndromes (1). DSCAM-L1 mediates homophilic adhesion and is involved in the formation of lamina-specific synaptic connections in the vertebrate retina (2).

References

  1. Agarwala, K.L. et al. (2001) Biochem. Biophys. Res. Commun. 285:760.
  2. Yamagata M., Sanes JR. (2008) Nature 451(7177):465.

Long Name

Down Syndrome Cell Adhesion Molecule Like 1

Alternate Names

DSCAML1

Entrez Gene IDs

57453 (Human); 114873 (Mouse); 315615 (Rat)

Gene Symbol

DSCAML1

UniProt

NP_065744

Additional DSCAM-L1 Products

Product Documents for Recombinant Human DSCAM-L1 Protein, CF

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Product Specific Notices for Recombinant Human DSCAM-L1 Protein, CF

For research use only

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