Recombinant Human DSCAM Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 3666-DS

R&D Systems, part of Bio-Techne
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3666-DS-050
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Key Product Details

Source

NS0

Accession #

O60469

Conjugate

Unconjugated

Applications

Bioactivity

View all DSCAM Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human DSCAM protein
Glu18-Met1595

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Glu18

Predicted Molecular Mass

175 kDa

SDS-PAGE

180-195 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated rhDSCAM in a functional ELISA with an apparent KD <10 nM.

Formulation, Preparation and Storage

3666-DS
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 400 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DSCAM

Down syndrome cell adhesion molecule (DSCAM) is a 220 kDa type I transmembrane glycoprotein and member of the immunoglobulin superfamily (1). Human DSCAM, which maps to a Down syndrome region of chromosome 21q22.2-22.3, is synthesized as a 2012 amino acid (aa) precursor that contains a 17 aa signal sequence, a 1578 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 396 aa cytoplasmic tail (SwissProt #: O60469). The ECD contains ten Ig-like C2-type domains, six fibronectin type-III domains, and 16 potential sites for N-linked glycosylation. Splicing variants lead to a second, shorter isoform, which has a ten aa substitution for aa 1562 - 1571 in the longer isoform, and a deletion of residues corresponding to aa 1572 - 2012 in the longer isoform. Human mature DSCAM is 98% aa identical to mature mouse and rat DSCAM. Studies on mice have shown that DSCAM is expressed widely in the developing nervous system (1 - 2). More recent studies indicate that DSCAM plays an important role in neurite arborization, cell body spacing, and lamina-specific synaptic targeting in vertebrate retina (2 - 4). DSCAM signaling in vertebrates is not well understood, but it has been shown that DSCAM directly binds to Pak1 and stimulates Pak1 phosphorylation and activity (5). In addition, DSCAM activates both JNK and p38 MAP kinases, and expression of the cytoplasmic domain of DSCAM induces a morphological change in cultured cells that is JNK-dependent (5). Thus, it appears that DSCAM signals through Pak1 and functions in axon guidance. Furthermore, DSCAM, in collaboration with DCC, interacts with netrin-1, and is a receptor required for netrin-dependent commissural axon outgrowth and pathfinding (2, 6).

References

  1. Yamakawa, K. et al. (1998) Hum. Mol. Genet. 7:227.
  2. Liu, G. et al. (2009) Proc. Natl. Acad. Sci. U.S.A. 106:2951.
  3. Fuerst, P.G. et al. (2008) Nature 451:470.
  4. Yamagata, M. and J.R. Sanes, 2008, Nature 451:465.
  5. Li, W. and K.-L. Guan (2004) J. Biol. Chem. 279:32824.
  6. Ly, A. et al. (2008) Cell 133:11241.

Long Name

Down Syndrome Cell Adhesion Molecule

Alternate Names

CHD2-42, CHD2-52

Entrez Gene IDs

1826 (Human); 13508 (Mouse)

Gene Symbol

DSCAM

UniProt

O60469

Additional DSCAM Products

Product Documents for Recombinant Human DSCAM Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human DSCAM Protein, CF

For research use only

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