Recombinant Human Elongin B/Elongin C/VHL Complex, CF

R&D Systems, part of Bio-Techne | Catalog # E3-600

R&D Systems, part of Bio-Techne
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E3-600-050
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

Q15370.1 (ELOB), Q15369.1 (ELOC), P40337.2 (VHL)

Conjugate

Unconjugated

Applications

Bioactivity

View all ELOB/ELOC/VHL Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human ELOB/ELOC/VHL Complex protein
Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Asp213 (VHL)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

24 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC)

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Formulation, Preparation and Storage

E3-600
Formulation Supplied as a solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: ELOB/ELOC/VHL Complex

Together, Elongin B (ELOB) and Elongin C (ELOC) form a heterodimer that serves as the regulatory subunit for the Elongin complex--a general transcription elongation factor that increases RNA Polymerase II transcription through template-encoded arresting sites.  The ELOB/ELOC complex also binds to the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  In this function, ELOB/ELOC serves as an adapter between substrate recognition proteins and either Cullin-2/Rbx1 (in VHL-box E3 Ubiquitin ligases) or Cullin-5/Rbx2 (in SOCS-box E3 Ubiquitin ligases).  VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta2 adrenergic receptor, ZHX2 and others.  

References

  1. Kamura T., et al.  (1998) Genes Dev. 12:3872.
  2. Okumura F., et al.  (2012) Front. Oncol. 2:10.
  3. Xie L., et al.  (2009) Sci. Signal. 2:ra33.
  4. Zhang J. et al.  (2018) Science 361:290. 

Long Name

Elongin B/Elongin C/VHL Complex

Alternate Names

Elongin B/Elongin C/VHL Complex

UniProt

Q15370.1 (ELOB), Q15369.1 (ELOC), P40337.2 (VHL)

Additional ELOB/ELOC/VHL Complex Products

Product Documents for Recombinant Human Elongin B/Elongin C/VHL Complex, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Elongin B/Elongin C/VHL Complex, CF

For research use only

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