Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF
R&D Systems, part of Bio-Techne | Catalog # E3-655
Key Product Details
Source
Sf 21 (baculovirus)
Accession #
Conjugate
Unconjugated
Applications
Bioactivity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex protein
Met1 - Asp213 (VHL), Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Ala 745 with a N-terminal 10-His tag Sumo tag (CUL2), Met1 - His108 (RBX1)
Met1 - Asp213 (VHL), Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Ala 745 with a N-terminal 10-His tag Sumo tag (CUL2), Met1 - His108 (RBX1)
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
25 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RBX1)
Activity
Typical protein concentration for use in vitro will depend on experimental conditions.
Reviewed Applications
Read 1 review rated 5 using E3-655 in the following applications:
Scientific Data Images for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF
Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1 SDS-PAGE
1 μg and 2 μg per lane of Recombinant Human VHL/ELOB/ELOC/CUL2/RBX1 Complex (Catalog # E3-655) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing bands corresponding to the individual proteins of the complex.Formulation, Preparation and Storage
E3-655
Formulation | Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol. |
Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: VHL/ELOB/ELOC/CUL2/RBX1 Complex
References
- Kamura T., et al. (1998) Genes & Dev. doi: 10.1101/gad.12.24.3872
- Okumura F., et al. (2012) Front. Oncol. doi: 10.3389/fonc.2012.00010
- Xie L., et al. (2009) Sci. Signaling doi: 10.1126/scisignal.2000444
- Zhang J., et al. (2018) Science doi: 10.1126/science.aap8411
Gene Symbol
VHL
Additional VHL/ELOB/ELOC/CUL2/RBX1 Complex Products
Product Documents for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF
Product Specific Notices for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF
For research use only
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