Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

R&D Systems, part of Bio-Techne | Catalog # E3-655

R&D Systems, part of Bio-Techne
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E3-655-025
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Key Product Details

Source

Sf 21 (baculovirus)

Accession #

P40337.2, Q15370.1, Q15369.1, Q13617.2, P62877.1

Conjugate

Unconjugated

Applications

Bioactivity

View all VHL/ELOB/ELOC/CUL2/RBX1 Complex Proteins and Enzymes »

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived human VHL/ELOB/ELOC/CUL2/RBX1 Complex protein
Met1 - Asp213 (VHL), Met1 - Gln118 (ELOB), Met1 - Cys112 (ELOC), Met1 - Ala 745 with a N-terminal 10-His tag Sumo tag (CUL2), Met1 - His108 (RBX1)

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Predicted Molecular Mass

25 kDa (VHL), 13.1 kDa (ELOB), 12.8 kDa (ELOC), 93 kDa (CUL2), 12 kDa (RBX1)

Activity

Typical protein concentration for use in vitro will depend on experimental conditions.

Reviewed Applications

Read 1 review rated 5 using E3-655 in the following applications:

Scientific Data Images for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1 SDS-PAGE

Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1 SDS-PAGE

1 μg and 2 μg per lane of Recombinant Human VHL/ELOB/ELOC/CUL2/RBX1 Complex (Catalog # E3-655) was resolved with SDS-PAGE under reducing conditions and visualized by colloidal Coomassie Blue staining, showing bands corresponding to the individual proteins of the complex.

Formulation, Preparation and Storage

E3-655
Formulation Supplied as a 0.2 μm filtered solution in HEPES, NaCl, DTT and Glycerol.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: VHL/ELOB/ELOC/CUL2/RBX1 Complex

VHL (von Hippel-Lindau disease tumor suppressor) is the substrate recognition subunit for an E3 ligase activity that ubiquitinates proteins containing hydroxyproline residues.  Targets of VHL include HIF1 alpha, beta2 adrenergic receptor, ZHX2 and others.  In VHL-box E3 Ubiquitin ligases, VHL is bound to a heterodimer complex composed of Elongin B (ELOB) and Elongin C (ELOC) through the "BC-box motif" found in many proteins in the VHL-box and SOCS-box protein families.  ELOB/ELOC serves as an adapter between substrate recognition proteins and Cullin-2 (or Cullin-5) and RBX1 to form an active E3 ligase.  

References

  1. Kamura T., et al. (1998) Genes & Dev.  doi: 10.1101/gad.12.24.3872
  2. Okumura F., et al. (2012) Front. Oncol.  doi: 10.3389/fonc.2012.00010
  3. Xie L., et al. (2009) Sci. Signaling  doi: 10.1126/scisignal.2000444
  4. Zhang J., et al. (2018) Science  doi: 10.1126/science.aap8411

Entrez Gene IDs

7428 (Human); 22346 (Mouse)

Gene Symbol

VHL

UniProt

P40337.2, Q15370.1, Q15369.1, Q13617.2, P62877.1

Additional VHL/ELOB/ELOC/CUL2/RBX1 Complex Products

Product Documents for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human Elongin B/Elongin C/VHL/CUL2/RBX1, CF

For research use only

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