Recombinant Human Endosialin/CD248 Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7855-CD

R&D Systems, part of Bio-Techne
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7855-CD-050
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Key Product Details

Source

NS0

Accession #

Q9HCU0

Conjugate

Unconjugated

Applications

Bioactivity

View all Endosialin/CD248 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human Endosialin/CD248 protein
Gln18-Arg685, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln18 predicted: No results obtained, sequencing might be blocked

Predicted Molecular Mass

72 kDa

SDS-PAGE

110-150 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human Endosialin/CD248 is immobilized at 2 μg/mL (100 μL/well), the concentration of Recombinant Human Galectin‑3BP/MAC‑2BP (Catalog # 2226-GA) that produces 50% of the optimal binding response is approximately 0.08-0.4 μg/mL.

Formulation, Preparation and Storage

7855-CD
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endosialin/CD248

Endosialin, also known as TEM1 (tumor endothelial marker 1) and designated CD248, is a 165‑175 kDa type I transmembrane O‑glycosylated protein that is expressed on activated perivascular and stromal cells in embyronic and tumor neovasculature, but down‑regulated in quiescent vasculature (1‑4). The human Endosialin cDNA encodes 757 amino acids (aa) including a 17 aa signal sequence, a 670 aa extracellular domain (ECD) with one C‑type lectin, one Sushi, one EGF‑like domain and a mucin-like stalk, a transmembrane sequence, and a short (49 aa) cytoplasmic domain with three potential phosphorylation sites and a PDZ binding motif (2, 3). Within the ECD, human Endosialin shares 76% and 74% aa sequence identity with mouse and rat Endosialin, respectively. Endosialin regulates pericyte proliferation, migration, and adhesion to endothelial cell‑deposited fibronectin and collagens I and IV in the extracellular matrix (3‑7). It binds Galectin‑3/MAC‑2BP, mediating a repulsive signal thought to guide tumor cell migration (5). In blood vessel development, it promotes endothelial cell apoptosis and pruning of unwanted capillaries (6). In humans, it is up‑regulated on mesenchymal stem cells, naïve CD8+ T cells, and leukocytes that express markers of endothelial and dendritic lineages (3, 8). It is dynamically expressed on lymphoid tissue stromal cells and is required for lymph node, spleen, and thymus remodeling during immune responses (8‑11). Its expression correlates with suppressed proliferation of human naïve CD8+ T cells, and with aggressiveness of some tumors (8, 12, 13). Endosialin expression is up‑regulated in vitro by high cell density and hypoxia (3, 14). Inflammatory conditions such as rheumatoid and psoriatic arthritis and IgA nephropathy can cause expression in stroma that do not normally express Endosialin (3).

References

  1. Rettig, W.J. et al. (1992) Proc. Natl. Acad. Sci. USA 89:10832.
  2. Christian, S. et al. (2001) J. Biol. Chem. 276:7408.
  3. Valdez, Y. et al. (2012) Curr. Drug Targets 13:432.
  4. MacFadyen, J.R. et al. (2005) FEBS Lett. 579:2569.
  5. Becker, R. et al. (2008/) FASEB J. 22:3059.
  6. Simonavicius, N. et al. (2012) Blood 120:1516.
  7. Tomkowicz, B. et al. (2010) Cancer Biol. Ther. 9:908.
  8. Hardie, D.L. et al. (2011) Immunology 133:288.
  9. Lax, S. et al. (2007) FEBS Lett. 581:3551.
  10. Lax, S. et al. (2010) Eur. J. Immunol. 40:1884.
  11. Lax, S. et al. (2012) FEBS Open Bio 2:187.
  12. Nanda, A. et al. (2006) Proc. Natl. Acad. Sci. USA 103:3351.
  13. Maia, M. et al. (2011) BMC Cancer 11:162.
  14. Ohradanova, A. et al. (2008) Br. J. Cancer 99:1348.

Alternate Names

CD164L1, CD248, TEM1

Entrez Gene IDs

57124 (Human); 70445 (Mouse); 293669 (Rat)

Gene Symbol

CD248

UniProt

Q9HCU0 (Human)

Additional Endosialin/CD248 Products

Product Documents for Recombinant Human Endosialin/CD248 Protein, CF

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Product Specific Notices for Recombinant Human Endosialin/CD248 Protein, CF

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