Recombinant Human EpCAM/TROP1 Avi-tag His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI9277

Biotinylated
R&D Systems, part of Bio-Techne
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AVI9277-050
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Key Product Details

Source

CHO

Accession #

P16422.2

Structure / Form

Biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all EpCAM/TROP1 Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human EpCAM/TROP1 protein
Human EpCAM
(Gln24-Lys265)
Accession # P16422.2		 Avi-tag 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24

Predicted Molecular Mass

31 kDa

SDS-PAGE

35-42 kDa, under reducing conditions.

Activity

Measured by its binding ability in a functional ELISA.
Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag (Catalog # AVI9277) binds to Human EpCAM/TROP-1 Antibody (Catalog # AF960) with a ED50 of 0.750-7.50 ng/mL.

Scientific Data Images for Recombinant Human EpCAM/TROP1 Avi-tag His-tag Protein, CF

Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag Protein Binding Activity.

Measured by its binding ability in a functional ELISA. Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag (Catalog # AVI9277) binds to Human EpCAM/TROP-1 Antibody (AF960) with a ED50 of 0.750-7.50 ng/mL.

Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag Protein SDS-PAGE.

2 μg/lane of Biotinylated Recombinant Human EpCAM/TROP‑1 Avi-tag His-tag Protein (Catalog # AVI9277) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-42 kDa.

Formulation, Preparation and Storage

AVI9277
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16). Our Avi-tag Biotinylated Recombinant Human EpCAM features biotinylation at a single site contained within the Avi-tag, a unique 15 amino acid peptide. Protein orientation will be uniform when bound to streptavidin-coated surface due to the precise control of biotinylation and the rest of the protein is unchanged so there is no interference in the protein's bioactivity.

References

  1. Strnad, J. et al. (1989) Cancer Res. 49:314.
  2. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
  3. Ng, V.Y. et al. (2010) Stem Cells 28:29.
  4. Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
  5. Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
  6. de Boer, C.J. et al. (1999) J. Pathol. 188:201.
  7. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
  8. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  9. Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
  10. Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
  11. Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
  12. Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
  13. Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
  14. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
  15. Schon, M.P. et al. (1993) Int. J. Cancer 55:988.
  16. Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

UniProt

P16422.2

Additional EpCAM/TROP1 Products

Product Documents for Recombinant Human EpCAM/TROP1 Avi-tag His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human EpCAM/TROP1 Avi-tag His-tag Protein, CF

For research use only

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