Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # AVI960

Biotinylated
R&D Systems, part of Bio-Techne
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AVI960-050
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Key Product Details

Learn more about Avi-tag Biotinylated Proteins

Source

HEK293

Accession #

P16422.2

Structure / Form

Disulfide-linked homodimer, biotinylated via Avi-tag

Conjugate

Biotin

Applications

Bioactivity

View all EpCAM/TROP1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human EpCAM/TROP1 protein

Human EpCAM/TROP-1
(Gln24-Lys265)
Accession # P16422.2

 IEGRMD Human IgG1
(Pro100-Lys330)		 Avi-tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln24, inferred from deblocking reaction revealing Glu25.

Predicted Molecular Mass

56 kDa

SDS-PAGE

55-75 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Human EpCAM/TROP1 Affinity Purified Polyclonal Antibody (Catalog # AF960) is immobilized at 0.25 µg/mL (100 µL/well), it binds to Biotinylated Recombinant Human EpCAM/TROP‑1 Fc Chimera Avi-tag with an ED50 of 1-6 ng/mL.

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.
The ED50 for this effect is 0.7-2.8 μg/mL.

Scientific Data Images for Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein, CF

Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein Binding Activity

Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein Binding Activity

When Human EpCAM/TROP1 Affinity Purified Polyclonal Ab (Catalog # AF960) is immobilized at 0.25 µg/mL (100 µg/well), Biotinylated Recombinant Human EpCAM/TROP-1 Fc Chimera Avi-tag (Catalog # AVI960) binds with an ED50 of 1-6 ng/mL.
Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein SDS-PAGE

Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein SDS-PAGE

2 μg/lane of Biotinylated Recombinant Human EpCAM/TROP-1 Fc Chimera Avi-tag (Catalog # AVI960) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 55-75 kDa and 110-150 kDa, respectively.

Formulation, Preparation and Storage

AVI960
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a transmembrane glycoprotein originally identified as a tumor-associated antigen due to its high expression level in rapidly growing epithelial tumors (1). EpCAM is one of several cell adhesion molecules (CAMs) that does not share the structural patterns found in the four major CAM families. Human EpCAM is composed of an extracellular domain (ECD) with two epidermal-growth-factor-like (EGF‑like) repeats, a single transmembrane domain, and a highly charged, short cytoplasmic domain. Within the mature ECD, human EpCAM shares 81% and 82% amino acid sequence identity with mouse and rat EpCAM respectively. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells (2). In adults, human EpCAM is detected in basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia, but is not detected in normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). EpCAM expression has been found to increase in actively proliferating epithelia tissues and in human malignant neoplasias arising from squamousal epithelia (2, 3). It has been found that EpCAM is present on normal hepatic stem cells and is increased on cells with advanced cirrhosis due to the cells' proliferation through autocrine activation of Wnt signaling (4). Additionally, EpCAM may be beneficial in the early diagnosis of hepatocellular carcinoma (HCC) since HCC typically originates from a cirrhotic background (4). EpCAM has been shown function as a homophilic Ca2+ independent adhesion molecule (3). Homophilic adhesion via EpCAM requires the interaction of both EGF­like repeats, with the first EGF­like repeat mediating reciprocal interaction between EpCAM molecules on opposing cells, while the second repeat is involved in lateral interaction of EpCAM. Lateral interaction of EpCAM lead to the formation of dimers and tetramers (5). During homophilic adhesion the cytoplasmic tail of EpCAM interacts with the actin cytoskeleton via a direct association alpha­actinin (6).

References

  1. Kloudova, K. et al. (2016) Oncotarget. 7:46120.
  2. Huang, L. et al. (2018) Int. J. Mol. Med. 42:1771.
  3. Munz, M. et al. (2009). J. Cancer Res. 69:5627.
  4. Khosla, R. et al. (2017). Stem Cells Trans. Med. 6:807.
  5. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  6. Balzar, M. et al. (1998) Mol. Cell. Biol. 18:4388.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

UniProt

P16422.2

Additional EpCAM/TROP1 Products

Product Documents for Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein, CF

Product Datasheets

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Product Specific Notices for Recombinant Human EpCAM/TROP1 Fc Chimera Avi-tag Protein, CF

For research use only

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