Recombinant Human EpCAM/TROP1 His-tag Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9277-EP

R&D Systems, part of Bio-Techne
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9277-EP-050
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Key Product Details

Source

HEK293

Accession #

P16422

Conjugate

Unconjugated

Applications

Bioactivity

View all EpCAM/TROP1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human EpCAM/TROP1 protein
Gln24-Lys265, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Gln24 inferred from enzymatic pyroglutamate treatment revealing Glu25

Predicted Molecular Mass

28 kDa

SDS-PAGE

30-42 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of the L Cells mouse fibroblast cell line.
When 5 x 104 cells/well are added to Recombinant Human EpCAM/TROP-1 and Human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is enhanced in a dose dependent manner. The ED50 for this effect is 0.4-2.4 μg/mL.

Scientific Data Images for Recombinant Human EpCAM/TROP1 His-tag Protein, CF

Recombinant Human EpCAM/TROP1 His-tag Protein Bioactivity

Recombinant Human EpCAM/TROP1 His-tag Protein Bioactivity

Immobilized Recombinant Human EpCAM/TROP1 (Catalog # 9277-EP) enhances L Cells mouse fibroblasts adhesion in the presence of Human Fibronectin (Catalog # 1918-FN). The ED50 for this effect is 0.4-2.4 µg/mL.

Formulation, Preparation and Storage

9277-EP
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 500 μg/mL in PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EpCAM/TROP1

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain (1). Human and mouse EpCAM share 82% aa sequence identity. During embryonic development, EpCAM is detected in fetal lung, kidney, liver, pancreas, skin, and germ cells. In adults, human EpCAM is expressed on basolateral cell membranes of all simple, pseudo-stratified, and transitional epithelia but not on normal squamous stratified epithelia, mesenchymal tissue, muscular tissue, neuro-endocrine tissue, or lymphoid tissue (2). It is additionally expressed on undifferentiated embryonic stem cells, thymocytes, and dendritic cells (3-5). It is up-regulated on actively proliferating epithelial tissues, during adult liver regeneration, and on many epithelial cell-derived carcinomas (2, 6). EpCAM functions as a homophilic cell adhesion molecule (7). It associates into tetramers and forms complexes in cis with Claudin-7, CD44v6, TSPAN8, CD9, Integrin alpha 3, and Annexin A1 (8-11) that can interfere with cell adhesion (12, 13). Proteolytic cleavage of EpCAM releases multiple fragments from the ECD as well as a cytoplasmic fragment that can regulate gene transcription (14-16).

References

  1. Strnad, J. et al. (1989) Cancer Res. 49:314.
  2. Schnell, U. et al. (2013) Biochim. Biophys. Acta 1828:1989.
  3. Ng, V.Y. et al. (2010) Stem Cells 28:29.
  4. Nelson, A.J. et al. (1996) Eur. J. Immunol. 26:401.
  5. Borkowski, T.A. et al. (1996) Eur. J. Immunol. 26:110.
  6. de Boer, C.J. et al. (1999) J. Pathol. 188:201.
  7. Litvinov, S.V. et al. (1994) J. Cell Biol. 125:437.
  8. Balzar, M. et al. (2001) Mol. Cell. Biol. 21:2570.
  9. Nubel, T. et al. (2009) Mol. Cancer Res. 7:285.
  10. Kuhn, S. et al. (2007) Mol. Cancer Res. 5:553.
  11. Schmidt, D.S. et al. (2004) Exp. Cell Res. 297:329.
  12. Litvinov, S.V. et al. (1997) J. Cell Biol. 139:1337.
  13. Gaiser, M.R. et al. (2012) Proc. Natl. Acad. Sci. USA 109:E889.
  14. Schnell, U. et al. (2013) Biosci. Rep. 33:e00030.
  15. Schon, M.P. et al. (1993) Int. J. Cancer 55:988.
  16. Maetzel, D. et al. (2009) Nat. Cell Biol. 11:162.

Long Name

Epithelial Cell Adhesion Molecule

Alternate Names

17-1A, CD326, GA733-2, gp40, KS1/4, M4S1, TACSTD1, TROP1

Entrez Gene IDs

4072 (Human); 17075 (Mouse); 171577 (Rat); 102123042 (Cynomolgus Monkey)

Gene Symbol

EPCAM

UniProt

P16422

Additional EpCAM/TROP1 Products

Product Documents for Recombinant Human EpCAM/TROP1 His-tag Protein, CF

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Recombinant Human EpCAM/TROP1 His-tag Protein, CF

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