Recombinant Human EphA1 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 7146-A1

R&D Systems, part of Bio-Techne
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7146-A1-100
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Key Product Details

Source

HEK293

Accession #

AAA36747

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all EphA1 Proteins and Enzymes »

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived human EphA1 protein
Human EphA1
(Met1-Glu547)
Accession # AAA36747		 DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys26

Predicted Molecular Mass

83.5 kDa (monomer)

SDS-PAGE

100-110 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EphA1 Fc Chimera is coated at 2 μg/mL (100 μL/well), the concentration of Biotinylayed Recombinant Mouse Ephrin‑A1 Fc Chimera (Catalog # BT602) that produces 50% of the optimal binding response is typically 10-50 ng/mL.

Formulation, Preparation and Storage

7146-A1
Formulation Supplied as a 0.2 μm filtered solution in MES, NaCl, PEG 3350, CHAPS and Imidazole.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: EphA1

EphA1, also known as Eph and Esk, is a 120‑130 kDa glycosylated member of the Eph family of transmembrane receptor tyrosine kinases. The A and B classes of Eph proteins are distinguished by Ephrin ligand binding preference but have a common structural organization. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1‑3). Mature mouse EphA1 consists of a 524 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 408 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains. The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) (4). Within the ECD, human EphA1 shares 84% aa sequence identity with mouse and rat EphA1. EphA1 can form pH sensitive cis-homodimers on the cell surface (5). Membrane-bound or clustered Ephrin ligands interact with EphA1 and activate its kinase domain which is capable of Ser, Thr, and Tyr phosphorylation (6). Reverse signaling is propagated through the Ephrin ligand (7). EphA1 is widely expressed in differentiated epithelial cells, particularly in bone marrow, spleen, thymus, and testes (6, 8). It is additionally expressed on CD4+ T cells and a subpopulation of CD19+ B cells (9, 10). On CD4+ T cells, EphA1 interacts with EphA4, induces Tyr phosphorylation of PYK2, and promotes chemokine-induced chemotaxis (9, 10). EphA1 is up‑regulated or down‑regulated in a variety of human carcinomas and is implicated in tumor invasiveness (3, 7, 11). The region of Fibronectin including type I repeats #10‑12 interacts with EphA1, and this interaction plays a role in VEGF-dependent in vitro angiogenesis (12).

References

  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  3. Mosch, B. et al. (2010) J. Oncol. Mar 10 Epub.
  4. Hirai H. et al. (1987) Science 238:1717.
  5. Bocharov, E.V. et al. (2008) J. Biol. Chem. 283:29385.
  6. Douville, E.M.J. et al. (1992) Mol. Cell. Biol. 12:2681.
  7. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  8. Coulthard, M.G. et al. (2001) Growth Factors 18:303.
  9. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  10. Holen, H.L. et al. (2010) J. Leukoc. Biol. 87:1059.
  11. Dong, Y. et al. (2009) Mod. Pathol. 22:151.
  12. Masuda, J. et al. (2008) J. Biol. Chem. 283:13148.

Alternate Names

Esk

Entrez Gene IDs

2041 (Human); 13835 (Mouse)

Gene Symbol

EPHA1

UniProt

AAA36747

Additional EphA1 Products

Product Documents for Recombinant Human EphA1 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human EphA1 Fc Chimera Protein, CF

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