Recombinant Human EphB2 Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 5189-B2

R&D Systems, part of Bio-Techne
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5189-B2-050
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Key Product Details

Source

NS0

Accession #

P29323

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

View all EphB2 Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived human EphB2 protein
Human EphB2
(Val19-Leu543)
Accession # P29323		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val19

Predicted Molecular Mass

84.7 kDa (monomer)

SDS-PAGE

103-108 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human EphB2 Fc Chimera is coated at 2 μg/mL, Recombinant Human Ephrin-B2 Fc Chimera (Catalog # 7397-EB) binds with an apparent Kd <0.1 nM.

Formulation, Preparation and Storage

5189-B2
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

Reconstitution Buffer Available:
Size / Price
Qty
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphB2

EphB2, also known as Cek5, Nuk, Erk, Qek5, Tyro5, Sek3, Hek5, and Drt, is a 125 kDa member of the transmembrane Eph receptor tyrosine kinase family that binds members of the Ephrin family on adjacent cells. The interaction triggers forward signaling in the receptor-expressing cells through the Eph receptor and reverse signaling in the ligand-expressing cells through Ephrin (1, 2). Human EphB2 cDNA encodes a 1055 amino acid (aa) precursor, which includes an 18 aa signal sequence, a 525 aa extracellar domain (ECD), a 21 aa transmembrane segment, and a 491 aa cytoplamic domain. The ECD contains a cysteine-rich region followed by two fibronectin type III domains. The cytoplasmic domain contains the tyrosine kinase domain, a sterile alpha motif (SAM), and a PDZ binding motif (3). Human EphB2 shares 99% aa sequence identity with mouse and rat EphB2 within the ECD region. A short isoform that lacks 70 aa at the C-terminus has also been reported (4). Hippocampal neurons can release vesicles containing full length EphB2, and these are taken up by neighboring glial cells (5). EphB2 is expressed on both sides of the neuronal synapse. It controls axon guidance across the embryonic midline, promotes a neuronal fate from neural precursors, and regulates NMDA receptor activity
(6-10). EphB2 interaction with Ephrin-A5 promotes axonal growth cone collapse, while its interaction with Ephrin-B ligands is required for inner ear, renal, urorectal, and vascular development (6, 11-15). Signaling in Ephrin expressing cells through EphB2-Ephrin complex requires proteolytic cleavage of EphB2 that releases its extracellular domain (16). Following the shedding of the extracellular domain of EphB2, the cytoplasmic domain of EphB2 is released from the plasma membrane by the presenilin-dependent gamma-secretase activity to initiate a signaling cascade in the EphB2 expressing cells (16). Aberrant EphB2 expression and activity are implicated in the progression of several cancers (17).

References

  1. Pasquale, E.B. (2008) Cell 133:38.
  2. Merlos-Suarez, A. and E. Batlle (2008) Curr. Opin. Cell Biol. 20:194.
  3. Fox, G.M. et al. (1995) Oncogene 10:897.
  4. Tang, X.X. et al. (1998) Oncogene, 17:521.
  5. Lauterbach, J. and R. Klein (2006) J. Neurosci. 26:11575.
  6. Cowan C.A. et al. (2000) Neuron 26:417.
  7. Bouvier, D. et al. (2008) J. Neurochem. 106:682.
  8. Cramer, K.S. et al. (2006) Dev. Biol. 295:76.
  9. Katakowski, M. et al. (2005) Neurosci. Lett. 385:204.
  10. Henderson, J.T. et al. (2001) Neuron 32:1041.
  11. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
  12. Dravis, C. et al. (2007) Hear. Res. 223:93.
  13. Dravis, C. et al. (2004) Dev. Biol. 271:272.
  14. Ogawa, K. et al. (2006) J. Cell Sci. 119:559.
  15. Salvucci, O. et al. (2006) Blood 108:2914.
  16. Litterst, C. et al. (2007) J. Biol. Chem. 282:16155.
  17. Castano, J. et al. (2008) Histol. Histopathol. 23:1011.

Long Name

Eph Receptor B2

Alternate Names

Cek5, Drt, Erk, Hek5, Nuk, Qek2, Sek3, Tyro5

Entrez Gene IDs

2048 (Human); 13844 (Mouse)

Gene Symbol

EPHB2

UniProt

P29323

Additional EphB2 Products

Product Documents for Recombinant Human EphB2 Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human EphB2 Fc Chimera Protein, CF

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