Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 10170-SG

R&D Systems, part of Bio-Techne
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10170-SG-050
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Key Product Details

Source

CHO

Accession #

O43556-1

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

View all epsilon-Sarcoglycan Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human epsilon-Sarcoglycan protein
Human epsilon-Sarcoglycan
(Asp47-Phe317)
Accession # O43556-1		 IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Asp47

Predicted Molecular Mass

58 kDa

SDS-PAGE

57-71 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of RT4-D6P2T rat schwannoma cells.
The ED50 for this effect is 0.8-4.8 μg/mL.

Scientific Data Images for Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF

Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein Bioactivity

Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein Bioactivity

Recombinant Human epsilon‑Sarcoglycan Fc Chimera (Catalog # 10170-SG) supports the adhesion of RT4‑D6P2T rat schwannoma cells. The ED50 for this effect is 0.8-4.8 μg/mL.
Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein SDS-PAGE

Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Human epsilon-Sarcoglycan Fc Chimera (Catalog # 10170-SG) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 57-71 kDa and 110-140 kDa, respectively.

Formulation, Preparation and Storage

10170-SG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: epsilon-Sarcoglycan

SGCE, or Epsilon-Sarcoglycan, is a type 1 transmembrane glycoprotein which was found as a homologue of alpha-sarcoglycan (1, 2). Sarcoglycans are part of the dystrophin-associated glycoprotein complex (DGC). This multiprotein complex connects the actin cytoskeleton to the extracellular matrix in cardiac and skeletal muscles (3, 4). The sarcogylcan complex is a subcomplex within the DGC and is composed of several muscle-specific forms. alpha, beta, gamma and delta-sarcoglycans are expressed predominantly in striated and smooth muscles while epsilon-Sarcoglycan (SGCE) is expressed in a wide range of tissues, with the highest levels in heart and lung (1-2, 5). Mutations in the human SGCE gene have been shown to be associated with myoclonus-dystonia (6). Human SGCE is synthesized as a 437 amino acid (aa) protein that includes a 317 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 99 aa cytoplasmic domain. Within the ECD, human SGCE shares 95% and 66% aa sequence identity the mouse and rat SGCE, respectively. There are multiple isoforms of SGCE protein present in humans. SGCE exon 11b isoform is differentially expressed in the brain (1). Knockout mutations in this isoform result in psychiatric diseases (7).

References

  1. Ettinger, A.J. et al. (1997) J. Biol. Chem. 272:32534.
  2. McNally, E.M. et al. (1998) FEBS Lett. 422:27.
  3. Blake, D.J. et al. (2002) Physiol. Rev. 82:291.
  4. Xiao, J. et al. (2017) Neurobiol. Dis. 98:52.
  5. Nishiyama, A. et al. (2004) Brain Res. Mol. Brain Res. 125:1.
  6. Zimprich, A. et al. (2001) Nat. Genet. 29:66.
  7. Peall. K.J. et al. (2013) Brain. 136:294.

Alternate Names

Sarcoglycan epsilon, SGCE

Entrez Gene IDs

8910 (Human); 20392 (Mouse); 432360 (Rat)

Gene Symbol

SGCE

UniProt

O43556-1

Additional epsilon-Sarcoglycan Products

Product Documents for Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF

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Product Specific Notices for Recombinant Human epsilon-Sarcoglycan Fc Chimera Protein, CF

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